DICER1 anticorps (AA 1-195)

N° du produit ABIN3043347

Détail du produit anti-DICER1 anticorps

Antigène: DICER1 (Dicer 1, Ribonuclease Type III (DICER1))

Épitope: AA 1-195

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DICER1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Fonction: Rabbit IgG polyclonal antibody for Endoribonuclease Dicer(DICER1) detection. Tested with WB, IHC-P in Human.

Réactivité croisée (Details): No cross reactivity with other proteins.

Attributs du produit: Rabbit IgG polyclonal antibody for Endoribonuclease Dicer(DICER1) detection. Tested with WB, IHC-P in Human.
Gene Name: dicer 1, ribonuclease type III
Protein Name: Endoribonuclease Dicer

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human Dicer recombinant protein (Position: M1-N195). Human Dicer shares 94% amino acid (aa) sequence identity with mouse Dicer.

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for Dicer is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Références pour anticorps anti-DICER1 (ABIN3043347)

Gao, Wang, Chen, Feng, Yang, Feng, Yang, Ye, Zhang: "Post-transcriptional modulation of protein phosphatase PPP2CA and tumor suppressor PTEN by endogenous siRNA cleaved from hairpin within PTEN mRNA 3'UTR in human liver cells." dans: Acta pharmacologica Sinica, Vol. 37, Issue 7, pp. 898-907, (2017) (PubMed).

Gao, Wang, Feng, Feng, Zheng, Yang, Xiao, Lu, Ye, Zhang: "A hairpin within YAP mRNA 3'UTR functions in regulation at post-transcription level." dans: Biochemical and biophysical research communications, Vol. 459, Issue 2, pp. 306-312, (2015) (PubMed).

Détails sur DICER1

Antigène: DICER1 (Dicer 1, Ribonuclease Type III (DICER1))

Autre désignation: DICER1 (DICER1 Produits)

Synonymes: anticorps dcr1, anticorps dicer, anticorps herna, anticorps DICER1, anticorps dicer1, anticorps 1110006F08Rik, anticorps D12Ertd7e, anticorps mKIAA0928, anticorps DCR1, anticorps Dicer, anticorps HERNA, anticorps MNG1, anticorps DICER, anticorps fc39d11, anticorps fc74b05, anticorps wu:fc39d11, anticorps wu:fc74b05, anticorps dicer 1, ribonuclease III, anticorps endoribonuclease Dicer, anticorps dicer 1, ribonuclease III S homeolog, anticorps dicer 1, ribonuclease type III, anticorps dicer 1 ribonuclease III, anticorps DICER1, anticorps CpipJ_CPIJ010534, anticorps dicer1, anticorps dicer1.S, anticorps Dicer1

Sujet: Dicer(DICER1), also known as endoribonuclease Dicer or helicase with RNase motif, is an enzyme that in humans is encoded by the DICER1 gene. It is mapped to 14q32.13. The DICER1 gene, a member of the ribonuclease III (RNaseIII) family, is involved in the generation of microRNAs (miRNAs), which modulate gene expression at the posttranscriptional level. DICER1 possesses an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus DICER, also known as helicase-MOI, is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. In addition, DICER1 is required for formation of the RNA induced silencing complex (RISC). It also cleaves double-stranded RNA to produce short interfering RNAs (siRNAs) which target the selective destruction of complementary RNAs.

Synonyms: DCR antibody|DCR1 antibody|Dcr1 homolog (Drosophila) antibody|Dicer 1 antibody|Dicer 1 ribonuclease type III antibody|Dicer antibody|DICER_HUMAN antibody|Dicer1 antibody|Double-strand-specific ribonuclease antibody|Endoribonuclease Dicer antibody|Helicase moi antibody|Helicase with RNAse motif antibody|HERNA antibody|KIAA0928 antibody|MNG1 antibody|RMSE2 antibody

ID gène: 23405

Pathways: Regulatory RNA Pathways, Stem Cell Maintenance, Ribonucleoprotein Complex Subunit Organization