POU2F1 anticorps (AA 11-240)
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- Antigène Voir toutes POU2F1 Anticorps
- POU2F1 (POU Domain, Class 2, Transcription Factor 1 (POU2F1))
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Épitope
- AA 11-240
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Reactivité
- Humain
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp POU2F1 est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Fonction
- Rabbit IgG polyclonal antibody for POU domain, class 2, transcription factor 1(POU2F1) detection. Tested with WB, IHC-P in Human.
- Réactivité croisée (Details)
- No cross reactivity with other proteins.
- Attributs du produit
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Rabbit IgG polyclonal antibody for POU domain, class 2, transcription factor 1(POU2F1) detection. Tested with WB, IHC-P in Human.
Gene Name: POU class 2 homeobox 1
Protein Name: POU domain, class 2, transcription factor 1 - Purification
- Immunogen affinity purified.
- Immunogène
- E.coli-derived human Oct-1 recombinant protein (Position: S11-Q240). Human Oct-1 shares 96% and 99% amino acid (aa) sequence identity with mouse and rat Oct-1, respectively.
- Isotype
- IgG
- Top Product
- Discover our top product POU2F1 Anticorps primaire
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- Indications d'application
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for Oct-1 is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. - Commentaires
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Concentration
- 500 μg/mL
- Buffer
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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- Antigène
- POU2F1 (POU Domain, Class 2, Transcription Factor 1 (POU2F1))
- Autre désignation
- POU2F1 (POU2F1 Produits)
- Synonymes
- anticorps OCT1, anticorps OTF1, anticorps oct-1B, anticorps POU2F1, anticorps 2810482H01Rik, anticorps NF-A1, anticorps Oct-1, anticorps Oct1, anticorps Otf-1, anticorps Otf1, anticorps chOCT-1, anticorps oct-1, anticorps oct1, anticorps pou2f1r, anticorps fc07d06, anticorps wu:fc07d06, anticorps zgc:158360, anticorps POU class 2 homeobox 1, anticorps POU domain, class 2, transcription factor 1, anticorps POU class 2 homeobox 1 S homeolog, anticorps POU class 2 homeobox 1b, anticorps POU2F1, anticorps Pou2f1, anticorps pou2f1.S, anticorps pou2f1b
- Sujet
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POU domain, class 2, transcription factor 1, also known as OTF1 or OCT1, is a protein that in humans is encoded by the POU2F1 gene. The POU2F1 transcription factor was among the first identified members of the POU transcription factor family. This gene is mapped to 1q24.1 to q24.2. It has been found that the POU dimer from the POU2F1 gene formed on the palindromic OCT factor recognition element, or PORE (ATTTGAAATGCAAAT), could recruit the transcriptional coactivator OBF1 , whereas POU dimers formed on the consensus MORE (more PORE) (ATGCATATGCAT) or on MOREs from immunoglobulin heavy chain promoters (AT[G/A][C/A]ATATGCAA) failed to interact. POU domain proteins contain a bipartite DNA-binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA.
Synonyms: FLJ42836 antibody|NF A1 antibody|NF-A1 antibody|Oct 1 antibody|Oct 1B antibody|Oct-1 antibody|OCT1 antibody|Octamer binding protein 1 antibody|Octamer binding transcription factor 1 antibody|Octamer-binding protein 1 antibody|Octamer-binding transcription factor 1 antibody|OTF 1 antibody|OTF-1 antibody|OTF1 antibody|OTTHUMP00000032348 antibody|OTTHUMP00000032350 antibody|OTTHUMP00000032351 antibody|PO21 antibody|PO2F1 antibody|PO2F1_HUMAN antibody|POU class 2 homeobox 1 antibody|POU domain class 2 transcription factor 1 antibody|POU domain, class 2, transcription factor 1 antibody|POU2F1 antibody - ID gène
- 5451
- UniProt
- P14859
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