PKC delta anticorps (AA 1-160)

N° du produit ABIN3043360

Cet anticorps Lapin Polyclonal détecte spécifiquement PKC delta dans WB, IF et ICC. Il présente une réactivité envers Humain et a été mentionné dans 1 publication.

Aperçu rapide pour PKC delta anticorps (AA 1-160) (ABIN3043360)

Antigène: PKC delta (PKCd) (Protein Kinase C, delta (PKCd))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PKC delta est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Détail du produit anti-PKC delta anticorps

Épitope: AA 1-160

Fonction: Anti-PKC delta/PRKCD Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-PKC delta/PRKCD Antibody (ABIN3043360). Tested in IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human PKC delta recombinant protein (Position: M1-E160). Human PKC delta shares 95% and 93% amino acid (aa) sequence identity with mouse and rat PKC delta, respctively.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
1. Qu, Y., Misaghi, S., Izrael-Tomasevic, A., Newton, K., Gilmour, L. L., Lamkanfi, M., Louie, S., Kayagaki, N., Liu, J., Komuves, L., Cupp, J. E., Arnott, D., Monack, D., Dixit, V. M.Phosphorylation of NLRC4 is critical for inflammasome activation. Nature 490: 539-542, 2012. 2. Salzer, E., Santos-Valente, E., Klaver, S., Ban, S. A., Emminger, W., Prengemann, N. K., Garncarz, W., Mullauer, L., Kain, R., Boztug, H., Heitger, A., Arbeiter, K., Eitelberger, F., Seidel, M. G., Holter, W., Pollak, A., Pickl, W. F., Forster-Waldl, E., Boztug, K. B-cell deficiency and severe autoimmunity caused by deficiency of protein kinase C-delta. Blood 121: 3112-3116, 2013. 3. Tu, X., Joeng, K. S., Nakayama, K. I., Nakayama, K., Rajagopal, J., Carroll, T. J., McMahon, A. P., Long, F. Noncanonical Wnt signaling through G protein-linked PKC-delta activation promotes bone formation. Dev. Cell 12: 113-127, 2007.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Références pour anticorps anti-PKC delta (ABIN3043360)

Gan, Wang, Zhao, Wu, Yang, Peng: "5-(Bis(3-(2-hydroxyethyl)-1H-indol-2-yl)methyl)-2-hydroxybenzoic acid (BHIMHA): showing a strategy of designing drug to block lung metastasis of tumors." dans: Drug design, development and therapy, Vol. 10, pp. 711-21, (2016) (PubMed).

Détails sur PKC delta

Antigène: PKC delta (PKCd) (Protein Kinase C, delta (PKCd))

Autre désignation: PRKCD

Sujet:

Synonyms: Protein kinase C delta type,2.7.11.13,Tyrosine-protein kinase PRKCD,2.7.10.2,nPKC-delta,Protein kinase C delta type regulatory subunit,Protein kinase C delta type catalytic subunit,Sphingosine-dependent protein kinase-1,SDK1,PRKCD,

Tissue Specificity: Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis.

Background: Protein kinase C delta type, also called PKCD, is an enzyme that in humans is encoded by the PRKCD gene. The PRKCD gene encodes a member of the protein kinase C family, members of which are critical for regulation of cell survival, proliferation, and apoptosis. This gene is mapped to 3p21.1. Studies both in human and mice demonstrate that this kinase is involved inB cell signaling and in the regulation of growth, apoptosis, and differentiation of a variety of cell types. PRKCD is also regulated by phosphorylation on various tyrosine residues including Y311 (by SRC). It has been showed that PRKCD phosphorylates NLRC4 and that this phosphorylation is critical for inflammasome assembly. What's more, it is also a part of a noncanonical WNT signaling cascade.

Sequence Similarities: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.

Poids moléculaire: 77 kDa

ID gène: 5580

UniProt: Q05655

Pathways: Interferon-gamma Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, M Phase, G-protein mediated Events, Dicarboxylic Acid Transport, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, Lipid Metabolism