PKC gamma anticorps (C-Term)

N° du produit ABIN3043714

Cet anticorps Lapin Polyclonal détecte spécifiquement PKC gamma dans WB. Il présente une réactivité envers Humain, Souris et Rat et a été mentionné dans 2+ publications.

Aperçu rapide pour PKC gamma anticorps (C-Term) (ABIN3043714)

Antigène: PKC gamma (PRKCG) (Protein Kinase C, gamma (PRKCG))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PKC gamma est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-PKC gamma anticorps

Épitope: AA 665-685, C-Term

Fonction: Anti-PKC gamma/PRKCG Antibody Picoband®

Séquence: IDQADFQGFT YVNPDFVHPD A

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-PKC gamma/PRKCG Antibody (ABIN3043714). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminus of human PKC gamma, identical to the related rat and mouse sequences.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Rat, Human, Mouse
1. Saito N, Shirai Y (2003). "Protein kinase C gamma (PKC gamma): function of neuron specific isotype.". J. Biochem. 132 (5): 683-7. 2. Verbeek, D. S., Knight, M. A., Harmison, G. G., Fischbeck, K. H., Howell, B. W. : Protein kinase C gamma mutations in spinocerebellar ataxia 14 increase kinase activity and alter membrane targeting. Brain 128: 436-442, 2005.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Agent conservateur: Thimerosal (Merthiolate), Sodium azide

Précaution d'utilisation: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Références pour anticorps anti-PKC gamma (ABIN3043714)

Gan, Wang, Zhao, Wu, Yang, Peng: "5-(Bis(3-(2-hydroxyethyl)-1H-indol-2-yl)methyl)-2-hydroxybenzoic acid (BHIMHA): showing a strategy of designing drug to block lung metastasis of tumors." dans: Drug design, development and therapy, Vol. 10, pp. 711-21, (2016) (PubMed).

Zhang, Xu, Song, Yao, Ji: "Extracts from Salvia-Nelumbinis naturalis alleviate hepatosteatosis via improving hepatic insulin sensitivity." dans: Journal of translational medicine, Vol. 12, pp. 236, (2015) (PubMed).

Détails sur PKC gamma

Antigène: PKC gamma (PRKCG) (Protein Kinase C, gamma (PRKCG))

Autre désignation: PRKCG

Sujet:

Synonyms: Protein kinase C gamma type,PKC-gamma,2.7.11.13,PRKCG,PKCG,

Tissue Specificity: Expressed in Purkinje cells of the cerebellar cortex. .

Background: The gamma isotype of protein kinase C (PKC gamma) is a member of the classical PKC (cPKC) subfamily which is activated by Ca (2+) and diacylglycerol in the presence of phosphatidylserine. Physiologically, PKC gamma is activated by a mechanism coupled with receptor-mediated breakdown of inositol phospholipid as other cPKC isotypes such as PKC alpha and PKC beta. PKC gamma is expressed solely in the brain and spinal cord and its localization is restricted to neurons, while PKC alpha and PKC beta are expressed in many tissues in addition to the brain. Within the brain, PKC gamma is the most abundant in the cerebellum, hippocampus and cerebral cortex, where the existence of neuronal plasticity has been demonstrated. PKC gamma gene is mutated in spinocerebellar ataxia type 14 (SCA14). Verbeek et al. (2005) point out the specific alterations in mutant PKC gamma function that could lead to the selective neuronal degeneration of SCA14.

Sequence Similarities: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.

Poids moléculaire: 104 kDa

UniProt: P05129

Pathways: Signalisation WNT, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Myometrial Relaxation and Contraction, G-protein mediated Events, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGF Signaling