MAP2K3 anticorps (C-Term)

N° du produit ABIN3043874

L’anticorps Lapin Polyclonal anti-MAP2K3 a été validé pour WB, IHC, IF, ICC et FACS. Il convient pour détecter MAP2K3 dans des échantillons de Humain, Souris et Rat. Il y a 2+ publications disponibles.

Aperçu rapide pour MAP2K3 anticorps (C-Term) (ABIN3043874)

Antigène: MAP2K3 (Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MAP2K3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Détail du produit anti-MAP2K3 anticorps

Épitope: AA 311-347, C-Term

Fonction: Anti-MEK3/MAP2K3 Antibody Picoband®

Séquence: AERMSYLELM EHPFFTLHKT KKTDIAAFVK EILGEDS

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-MEK3/MAP2K3 Antibody Picoband® (ABIN3043874). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminus of human MEK3, identical to the related mouse sequence.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Rampoldi L, Zimbello R, Bortoluzzi S, Tiso N, Valle G, Lanfranchi G, Danieli GA (Mar 1998). "Chromosomal localization of four MAPK signaling cascade genes: MEK1, MEK3, MEK4 and MEKK5". Cytogenet Cell Genet 78 (3-4): 301-3.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Références pour anticorps anti-MAP2K3 (ABIN3043874)

Cheng, Wang, Wang, Wang, Du, Lou: "Silencing Ras-Related C3 Botulinum Toxin Substrate 1 Inhibits Growth and Migration of Hypopharyngeal Squamous Cell Carcinoma via the P38 Mitogen-Activated Protein Kinase Signaling Pathway." dans: Medical science monitor : international medical journal of experimental and clinical research, Vol. 24, pp. 768-781, (2018) (PubMed).

Xu, Zhang, Wei, Jia, Ge, Zhang, Liu: "MicroRNA-21 promotes hepatocellular carcinoma HepG2 cell proliferation through repression of mitogen-activated protein kinase-kinase 3." dans: BMC cancer, Vol. 13, pp. 469, (2013) (PubMed).

Détails sur MAP2K3

Antigène: MAP2K3 (Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3))

Autre désignation: MAP2K3

Sujet:

Synonyms: Dual specificity mitogen-activated protein kinase kinase 3,MAP kinase kinase 3,MAPKK 3,2.7.12.2,MAPK/ERK kinase 3,MEK 3,Stress-activated protein kinase kinase 2,SAPK kinase 2,SAPKK-2,SAPKK2,MAP2K3,MEK3, MKK3, PRKMK3, SKK2,

Tissue Specificity: Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.

Background: Dual specificity mitogen-activated protein kinase kinase 3 is an enzyme that in humans is encoded by the MAP2K3 gene. The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. And this kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. Rampoldi et al. (1997) localized the MAP2K3 gene to 17q11.2.

Poids moléculaire: 40 kDa

ID gène: 5606

UniProt: P46734

Pathways: Signalisation MAPK, Signalisation TLR, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling Events mediated by VEGFR1 and VEGFR2