Aquaporin 4 anticorps (C-Term)

N° du produit ABIN3044015

Détail du produit anti-Aquaporin 4 anticorps

Antigène: Aquaporin 4 (AQP4)

Épitope: AA 258-274, C-Term

Reactivité: Humain, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Aquaporin 4 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Fonction: Rabbit IgG polyclonal antibody for Aquaporin-4(AQP4) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Séquence: FKRRFKEAFS KAAQQTK

Réactivité croisée (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Attributs du produit: Rabbit IgG polyclonal antibody for Aquaporin-4(AQP4) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: aquaporin 4
Protein Name: Aquaporin-4

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 4(258-274aa FKRRFKEAFSKAAQQTK), different from the related rat and mouse sequences by two amino acids.

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, The detection limit for AQP4 is approximately 0.5 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Agent conservateur: Thimerosal (Merthiolate), Sodium azide

Précaution d'utilisation: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Date de péremption: 12 months

Références pour anticorps anti-Aquaporin 4 (ABIN3044015)

Han, Huang, Liu, Sha, Hu, Liu: "Changes in the Expression of AQP4 and AQP9 in the Hippocampus Following Eclampsia-Like Seizure." dans: International journal of molecular sciences, Vol. 19, Issue 1, (2018) (PubMed).

Wang, Bu, Zhang, Chen, Zhang, Bao: "Expression pattern of aquaporins in patients with primary nephrotic syndrome with edema." dans: Molecular medicine reports, Vol. 12, Issue 4, pp. 5625-32, (2016) (PubMed).

Zhang, Chen, Dang, Liu, Ito, Sun: "Neuroprotective effects of total steroid saponins on cerebral ischemia injuries in an animal model of focal ischemia/reperfusion." dans: Planta medica, Vol. 80, Issue 8-9, pp. 637-44, (2015) (PubMed).

Chen, Huang, Ruan, He, Li: "Ulinastatin attenuates cerebral ischemia-reperfusion injury in rats." dans: International journal of clinical and experimental medicine, Vol. 7, Issue 5, pp. 1483-9, (2014) (PubMed).

Détails sur Aquaporin 4

Antigène: Aquaporin 4 (AQP4)

Autre désignation: AQP4 (AQP4 Produits)

Synonymes: anticorps zgc:100922, anticorps si:ch211-192k9.1, anticorps AQP4, anticorps LOC100232616, anticorps HMIWC2, anticorps MIWC, anticorps WCH4, anticorps AQP-4, anticorps AQP4.M23, anticorps MIWC2, anticorps Miwc, anticorps aquaporin-4, anticorps aquaporin 4, anticorps aquaporin 4 L homeolog, anticorps aqp4, anticorps AQP4, anticorps aqp4.L, anticorps Aqp4

Sujet: AQP4(Aquaporin 4), also known as MERCURIAL-INSENSITIVE WATER CHANNEL, MIWC, is protein which in humans is encoded by the AQP4 gene. The aquaporins are a family of water-selective membrane channels found in animals, plants, and microorganisms. AQP4 is the predominant water channel in the brain and has an important role in brain water homeostasis. By fluorescence in situ hybridization, Lu et al.(1996) determined that the AQP4 gene maps to 18q11.2-q12.1. By interspecific backcross analysis, Turtzo et al.(1997) mapped the mouse Aqp4 gene to the proximal region of chromosome 18. Analyzing the expression of AQP4 in mammalian skeletal muscle, Frigeri et al.(1998) found that, in immunohistochemical experiments, affinity-purified AQP4 antibodies stained selectively the sarcolemma of fast-twitch fibers. Immunocytochemistry revealed strong AQP4 water channel expression in Muller cells in mouse retina and in fibrous astrocytes in optic nerve.

Synonyms: AQP 4 antibody|AQP-4 antibody|AQP4 antibody|AQP4_HUMAN antibody|Aquaporin type 4 antibody|Aquaporin-4 antibody|Aquaporin4 antibody|HMIWC 2 antibody|HMIWC2 antibody|Mercurial insensitive water channel antibody|Mercurial-insensitive water channel antibody|MGC22454 antibody|MIWC antibody|WCH 4 antibody|WCH4 antibody

UniProt: P55087

Pathways: Sensory Perception of Sound