PDPK1 anticorps (C-Term)

N° du produit ABIN3044043

Cet anticorps anti-PDPK1 est un anticorps Lapin Polyclonal détectant PDPK1 dans WB et IHC. Adapté pour Humain et Rat. Ce Primary Antibody a été cité dans 2+ publications.

Aperçu rapide pour PDPK1 anticorps (C-Term) (ABIN3044043)

Antigène: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Reactivité: Humain, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PDPK1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-PDPK1 anticorps

Épitope: AA 408-421, C-Term

Fonction: Anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 Antibody Picoband®

Séquence: WKHYNTNHEA DDWC

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 Antibody (ABIN3044043). Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminus of human Mitochondrial Pyruvate dehydrogenase kinase 1, different from the related rat sequence by one amino acid, and from the related mouse sequence by two amino acids.

Isotype: IgG

Information d'application

Indications d'application: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat
Western blot, 0.1-0.5 μg/mL, Human, Rat
1. Gudi, R., Bowker-Kinley, M. M., Kedishvili, N. Y., Zhao, Y., Popov, K. M. Diversity of the pyruvate dehydrogenase kinase gene family in humans. J. Biol. Chem. 270: 28989-28994, 1995.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Agent conservateur: Thimerosal (Merthiolate), Sodium azide

Précaution d'utilisation: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Références pour anticorps anti-PDPK1 (ABIN3044043)

Li, Zhao, Qi, Wang, Zhang, Li, Qin: "lncRNA Ftx promotes aerobic glycolysis and tumor progression through the PPARγ pathway in hepatocellular carcinoma." dans: International journal of oncology, Vol. 53, Issue 2, pp. 551-566, (2018) (PubMed).

Xu, Ding, Fang, Zheng, Gao, Lu, Zhang: "Silencing of HIF-1alpha suppresses tumorigenicity of renal cell carcinoma through induction of apoptosis." dans: Cancer gene therapy, Vol. 17, Issue 3, pp. 212-22, (2010) (PubMed).

Détails sur PDPK1

Antigène: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Autre désignation: PDK1

Sujet:

Synonyms: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial,2.7.11.2,Pyruvate dehydrogenase kinase isoform 1,PDH kinase 1,PDK1,PDHK1,

Tissue Specificity: Expressed predominantly in the heart. Detected at lower levels in liver, skeletal muscle and pancreas. .

Background: PDK1 (Pyruvate Dehydrogenase Kinase Isoenzyme 1), is an enzyme that in humans is encoded by the PDK1 gene. To find human PDKs, Gudi et al. (1995) used oligonucleotide primers to screen a human liver cDNA library by PCR. They identified and reported the deduced amino acid sequences of PDK1, PDK2, and PDK3. The human PDK1 gene encodes a protein with a predicted molecular mass of 49,244 Da that shares 93 % identity with that of the rat PDK1 gene. Northern blot analysis showed that the PDK1 message was expressed predominantly in the heart.

Sequence Similarities: Belongs to the PDK/BCKDK protein kinase family.

Poids moléculaire: 85-91 kDa

UniProt: Q15118

Pathways: Signalisation PI3K-Akt, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals