Vinculin anticorps (N-Term)

N° du produit ABIN3044168

Détail du produit anti-Vinculin anticorps

Antigène: Vinculin (VCL)

Épitope: AA 173-188, N-Term

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Vinculin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Fonction: Rabbit IgG polyclonal antibody for Vinculin(VCL) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Séquence: KMIDERQQEL THQEHR

Réactivité croisée (Details): No cross reactivity with other proteins.

Attributs du produit: Rabbit IgG polyclonal antibody for Vinculin(VCL) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: vinculin
Protein Name: Vinculin

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the N-terminus of human Vinculin(173-188aa KMIDERQQELTHQEHR), identical to the related rat and mouse sequences.

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Agent conservateur: Thimerosal (Merthiolate), Sodium azide

Précaution d'utilisation: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Date de péremption: 12 months

Références pour anticorps anti-Vinculin (ABIN3044168)

Cong, Fu, Zhang, Zhao, Zhang: "Effects of atorvastatin on porcine aqueous humour outflow and trabecular meshwork cells." dans: Experimental and therapeutic medicine, Vol. 15, Issue 1, pp. 210-216, (2018) (PubMed).

Wang, Wang, Qu, Yue, Hu, Zhang: "VEGF Enhances the Migration of MSCs in Neural Differentiation by Regulating Focal Adhesion Turnover." dans: Journal of cellular physiology, Vol. 230, Issue 11, pp. 2728-42, (2015) (PubMed).

Shen, Lu, Li, Zhang, Zhu: "Synthesis and characterization of biodegradable polyurethane for hypopharyngeal tissue engineering." dans: BioMed research international, Vol. 2015, pp. 871202, (2015) (PubMed).

Su, Liu, Ma, Wang, E, Liu: "Enhancement of periodontal tissue regeneration by transplantation of osteoprotegerin-engineered periodontal ligament stem cells." dans: Stem cell research & therapy, Vol. 6, pp. 22, (2015) (PubMed).

Lyu, Wang, Wang, Ding, Liu, Yuan, Shi, Wang, Wang, Chen: "Maintaining the pluripotency of mouse embryonic stem cells on gold nanoparticle layers with nanoscale but not microscale surface roughness." dans: Nanoscale, Vol. 6, Issue 12, pp. 6959-69, (2014) (PubMed).

Lyu, Hu, Xu, Zhang: "Dynamics of focal adhesions and reorganization of F-actin in VEGF-stimulated NSCs under varying differentiation states." dans: Journal of cellular biochemistry, Vol. 114, Issue 8, pp. 1744-59, (2013) (PubMed).

Détails sur Vinculin

Antigène: Vinculin (VCL)

Autre désignation: VCL (VCL Produits)

Synonymes: anticorps CMD1W, anticorps CMH15, anticorps MV, anticorps MVCL, anticorps VCL, anticorps im:6902790, anticorps hm:zehn2160, anticorps DDBDRAFT_0186762, anticorps DDBDRAFT_0232320, anticorps DDB_0186762, anticorps DDB_0232320, anticorps 9430097D22, anticorps AA571387, anticorps AI462105, anticorps AW545629, anticorps CG3299, anticorps CT11081, anticorps Dmel\\CG3299, anticorps Dmvincp, anticorps EG:103B4.1, anticorps Vin, anticorps Vin2EF, anticorps vinc, anticorps LOC100228062, anticorps vcl-A, anticorps vinculin, anticorps vinculin a, anticorps vinculin A, anticorps Vinculin, anticorps vinculin S homeolog, anticorps VCL, anticorps vcla, anticorps vinA, anticorps Vcl, anticorps Vinc, anticorps CpipJ_CPIJ013873, anticorps Bm1_37935, anticorps vcl, anticorps vcl.S

Sujet: Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.

Synonyms: CMD1W antibody|CMH15 antibody|Metavinculin antibody|MVCL antibody|OTTHUMP00000019861 antibody|OTTHUMP00000019862 antibody|VCL antibody|VINC antibody|VINC_HUMAN antibody|Vinculin antibody

UniProt: P18206

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2