DNMT1 anticorps (AA 637-650)

N° du produit ABIN305735

L’anticorps anti-DNMT1 Monoclonal Souris est utilisé pour la détection de DNMT1 dans des échantillons de Humain, Souris, Singe, Cheval, Lapin et Roussette (Chauve-souris). Il a été validé pour WB, IHC, IP, ChIP et IHC (p).

Aperçu rapide pour DNMT1 anticorps (AA 637-650) (ABIN305735)

Antigène: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Reactivité: Humain, Souris, Singe, Cheval, Lapin, Roussette (Chauve-souris)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp DNMT1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 60B1220-1

Détail du produit anti-DNMT1 anticorps

Épitope: AA 637-650

Specificité: A synthetic peptide corresponding to amino acids 637-650 (EKDDREDKENAFKR) of human Dnmt1 (Genbank Accession No. NP_001370). It will cross react with mouse Dnmt1.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Panda, Bat, Horse, Rabbit (100%) Sheep, Hamster, Elephant, Dog, Bovine, Pig (93%).

Purification: Protein G purified

Immunogène: A synthetic peptide corresponding to amino acids 637-650 (EKDDREDKENAFKR) of human Dnmt1 (Genbank Accession No. NP_001370). It will cross react with mouse Dnmt1. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Panda, Bat, Horse, Rabbit (100%), Sheep, Hamster, Elephant, Dog, Bovine, Pig (93%).

Type of Immunogen: Synthetic peptide

Isotype: IgG1

Information d'application

Indications d'application: Approved: ChrIP, IHC, IHC-P (5 μg/mL), IP, WB (2 - 4 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.05 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur DNMT1

Antigène: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Autre désignation: DNMT / DNMT1

Sujet: Name/Gene ID: DNMT1
Family: C5-methyltransferase

Synonyms: DNMT1, AIM, CXXC finger protein 9, CXXC9, DNA methyltransferase 1, DNMT, DNA methyltransferase HsaI, DNA MTase HsaI, HSN1E, M.HsaI, MCMT

ID gène: 1786

NCBI Accession: NP_001370

UniProt: P26358

Pathways: SARS-CoV-2 Protein Interactome, Phosphorylation & l'infection par le SRAS-CoV-2