Insulin Receptor anticorps

N° du produit ABIN317782

Cet anticorps Lapin Polyclonal détecte spécifiquement Insulin Receptor dans WB. Il présente une réactivité avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour Insulin Receptor anticorps (ABIN317782)

Antigène: Insulin Receptor (INSR)

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Insulin Receptor est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-Insulin Receptor anticorps

Specificité: IR antibody detects endogenous levels of IR protein.(region surrounding Tyr1355)

Purification: Affinity chromatography

Information d'application

Indications d'application: ELISA: 1: 1000approx. 1: 5000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1,0 mg/mL

Buffer: PBS, pH ~7.2, 0.05 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur Insulin Receptor

Antigène: Insulin Receptor (INSR)

Autre désignation: CD220 / INSR

Sujet: The Insulin Receptor (IR) is synthesized as a single polypeptide, which is subsequently cleaved to generate an extracellular α chain and a transmembrane and intracellular β chain, tethered together by disulfide bonds. The β chain has multiple tyrosine phosphorylation sites, including three autophosphorylation sites at its activation loop. Insulin stimulates the signaling cascade by binding to the insulin receptor through its extracellular α subunits. This stimulates the tyrosine kinase activity of the β subunits of the receptor. The receptor then autophosphorylates itself and phosphorylates the IRS (Insulin Receptor Substrate) proteins that are very important modulators of insulin signaling. Phosphorylated IRS, in turn, interacts with several downstream effectors of insulin signaling, including PI3 Kinase p85, GRB2, SHP2, Nck, Crk, and Fyn. The overall structure of the IR is highly homologous to the IGF-I Receptor, except in their c-termini, where the two proteins diverge somewhat. Insulin signaling is highly dependent on the PI3 Kinase pathway and Akt, which appear to mediate the functions of insulin.Synonyms: Insulin Receptor

Poids moléculaire: approx. 95, 156 kDa

ID gène: 3643

NCBI Accession: NP_000199

UniProt: P06213

Pathways: Signalisation NF-kappaB, Signalisation RTK, AMPK Signaling, Carbohydrate Homeostasis, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Growth Factor Binding, Negative Regulation of Transporter Activity