CHEK2 anticorps (pSer516)

N° du produit ABIN317990

L’anticorps anti-CHEK2 Polyclonal Lapin est utilisé pour la détection de CHEK2 dans des échantillons de Humain. Il a été validé pour WB.

Aperçu rapide pour CHEK2 anticorps (pSer516) (ABIN317990)

Antigène: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CHEK2 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-CHEK2 anticorps

Épitope: pSer516

Specificité: P-Chk2 (pSer516) antibody detects endogenous levels of p-Chk2 protein.

Purification: Affinity chromatography

Information d'application

Indications d'application: ELISA: 1: 10000approx. 1: 20000. Western Blot: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur CHEK2

Antigène: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Autre désignation: CHK2

Sujet: Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by proteolysis of cyclins. Chk1 and Chk2 are involved in these processes as regulators of Cdks. Chk1 and Chk2 both function as essential components in the G2 DNA damage checkpoint by phosphorylating Cdc25C in response to DNA damage. Phosphorylation inhibits Cdc25C activity, thereby blocking mitosis. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. It has also been shown that Chk1 can phosphorylate Wee1 in vitro, providing evidence that the hyper-phosphorylated form of Wee1, seen in cells delayed by Chk1 over-expression, is due to phosphorylation by Chk1. Chk1 is phosphorylated on Serine 345 (S345) in response to UV, IR and hydroxyurea (HU). Chk1 plays an essential role in the mammalian DNA damage checkpoint, embryonic development and tumor suppression.Synonyms: CHEK-2, CHEK2, CHK-2, CHK2 checkpoint homolog, Cds1, RAD53, Serine/threonine-protein kinase Chk2

Poids moléculaire: approx. 62 kDa

ID gène: 11200

NCBI Accession: NP_001005735

UniProt: O96017

Pathways: Signalisation p53, Apoptose, Cycle Cellulaire