Cathepsin G anticorps (Internal Region)

N° du produit ABIN3183694

Détail du produit anti-Cathepsin G anticorps

Antigène: Cathepsin G (CTSG)

Épitope: Internal Region

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Cathepsin G est non-conjugé

Application: Western Blotting (WB), ELISA

Specificité: Cathepsin G Polyclonal Antibody detects endogenous levels of Cathepsin G protein.

Attributs du produit: Rabbit Polyclonal to Cathepsin G.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogène: Synthesized peptide derived from the Internal region of human Cathepsin G.

Isotype: IgG

Information d'application

Indications d'application: WB 1:500-1:2000, ELISA 1:40000,

Restrictions: For Research Use only

Independent Validation (UP)

Validation #102721 (Unfolding Profile)

by: NanoTemper Technologies

No.: #102721

Date: 05.11.2018

Antigène: CTSG

Numéro du lot: 022204892

Application validée: Unfolding Profile

Contrôle positif: ABIN3183694

Conclusion:

Passed. ABIN3183694 showed T_i at 85.2°C and a clear unfolding profile with one unfolding event. This suggests that the protein is properly folded and functional.

Validation Images

p>Unfolding profile of ABIN3183694. The fluorescence signal is plotted against temperature. The native (folded) protein has a high signal at the beginning of the experiment, which decreases and then again increases upon unfolding, showing one unfolding event. The vertical line indicates the Ti at 85.2°C.

Protocole

Anticorps primaire: ABIN3183694

Full Protocol:

• Dilute ABIN3183694 1:10 in PBS buffer (Roth, 1058.1, lot 285231988) to get a final volume of 15µl at a concentration of 0.67µM.
• Load sample into Tycho capillary (NanoTemper Technologies, TY-C001).
• Run Tycho measurement.

Notes:

Tycho is designed to run quick and precise protein quality check experiments. Tycho uses intrinsic protein fluorescence to follow protein unfolding while running a fast thermal ramp, yielding results in 3min. A protein’s unfolding behavior is characterized by various parameters, most notably the inflection temperature (T_i). The T_i can be used to identify properly folded protein, to compare different batches, or to analyze the influence of storage/transport conditions on a protein. An absence of T_i would suggest that the protein is already unfolded and therefore most likely nonfunctional.

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles.

Stock: -20 °C

Stockage commentaire: Store at -20°C.

Détails sur Cathepsin G

Antigène: Cathepsin G (CTSG)

Autre désignation: Cathepsin G (CTSG Produits)

Synonymes: anticorps CATG, anticorps CG, anticorps CTSG, anticorps Ctsg, anticorps cathepsin G, anticorps CTSG, anticorps Ctsg, anticorps LOC505658, anticorps LOC509956, anticorps ctsg, anticorps LOC100053921, anticorps LOC102174648, anticorps LOC100733619, anticorps LOC100154047

Poids moléculaire: 22 kDa

ID gène: 1511

UniProt: P08311

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones