CDK6 anticorps (pTyr24)

N° du produit ABIN319290

Il existe 2+ publications pour ce produit. L’anticorps anti-CDK6 Polyclonal Lapin est utilisé pour la détection de CDK6 dans des échantillons de Humain et Souris. Il a été validé pour WB, IF et IHC (p).

Aperçu rapide pour CDK6 anticorps (pTyr24) (ABIN319290)

Antigène: CDK6 (Cyclin-Dependent Kinase 6 (CDK6))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CDK6 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-CDK6 anticorps

Épitope: pTyr24

Specificité: Antibody AP08040PU detects endogenous levels of CDK6 only when phosphorylated at Tyrosine 24.

Purification: Affinity Chromatography

Immunogène: Synthetic phosphopeptide derived from human CDK6 around the phosphorylation site of Tyrosine 24 (G-A-YP-G-K).

Information d'application

Indications d'application: Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin-Embedded Sections: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody (in aliquots) at-20 °C.

Références pour anticorps anti-CDK6 (ABIN319290)

Bychkov, Shulepko, Vasileva, Sudarikova, Kirpichnikov, Lyukmanova: "ASIC1a Inhibitor mambalgin-2 Suppresses the Growth of Leukemia Cells by Cell Cycle Arrest." dans: Acta naturae, Vol. 12, Issue 2, pp. 101-116, (2020) (PubMed).

Bychkov, Shulepko, Osmakov, Andreev, Sudarikova, Vasileva, Pavlyukov, Latyshev, Potapov, Kirpichnikov, Shenkarev, Lyukmanova: "Mambalgin-2 Induces Cell Cycle Arrest and Apoptosis in Glioma Cells via Interaction with ASIC1a." dans: Cancers, Vol. 12, Issue 7, (2020) (PubMed).

Détails sur CDK6

Antigène: CDK6 (Cyclin-Dependent Kinase 6 (CDK6))

Autre désignation: CDK6

Sujet: Cdk6 is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of Cdk6 first appears in mid-G1 phase, and is controlled by regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb.Synonyms: Cell division protein kinase 6, PLSTIRE, Serine/threonine-protein kinase PLSTIRE

ID gène: 1021

NCBI Accession: NP_001138778

UniProt: Q00534

Pathways: Cycle Cellulaire, Mitotic G1-G1/S Phases