Cofilin anticorps (Tyr88)

N° du produit ABIN319381

Cet anticorps Lapin Polyclonal détecte spécifiquement Cofilin dans WB, IF et IHC (p). Il présente une réactivité envers Humain et Souris.

Aperçu rapide pour Cofilin anticorps (Tyr88) (ABIN319381)

Antigène: Cofilin (CFL1) (Cofilin 1 (CFL1))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Cofilin est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-Cofilin anticorps

Épitope: Tyr88

Specificité: This antibody detects endogenous levels of total Cofilin-1/2 protein.

Réactivité croisée (Details): Also reacts to: Cofilin 2

Purification: Immunoaffinity Chromatography.

Immunogène: The antiserum was produced against synthesized non-phosphopeptide derived from human Cofilin1/Cofilin2 around the phosphorylation site of Tyrosine 88 (A-T-Yp-E-T).

Information d'application

Indications d'application: Western Blot: 1/500approx. 1/1000. Immunofluorescence: 1/100approx. 1/200. Immunohistochemistry on Paraffin-Embedded Sections: 1/50approx. 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody (in aliquots) at-20 °C.

Détails sur Cofilin

Antigène: Cofilin (CFL1) (Cofilin 1 (CFL1))

Autre désignation: Cofilin-1

Sujet: Cofilin is ubiquitously expressed in eukaryotic cells where it binds to Actin, thereby regulating the rapid cycling of Actin assembly and disassembly essential for cellular viability. Cofilin is a low molecular weight protein that binds to filamentous F-Actin by bridging two longitudinally associated Actin subunits changing the F-Actin filament twist. This process is allowed by the dephosphorylation of Cofilin Ser 3 by factors such as opsonized zymosan. Lim kinase 1, a serine kinase, phosphorylates Cofilin and renders it unable to bind and depolymerise F-Actin.Synonyms: 18 kDa phosphoprotein, CFL, CFL1, Cofilin non-muscle isoform, p18

ID gène: 1072

NCBI Accession: NP_005498

UniProt: P23528

Pathways: Regulation of Cell Size, Tube Formation, Protein targeting to Nucleus, CXCR4-mediated Signaling Events