CD11b anticorps

N° du produit ABIN319553

L’anticorps Rat Monoclonal anti-CD11b a été validé pour FACS, IP, IHC (fro), Func et IHC (p). Il convient pour détecter CD11b dans des échantillons de Humain, Souris et Lapin.

Aperçu rapide pour CD11b anticorps (ABIN319553)

Antigène: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reactivité: Humain, Souris, Lapin

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp CD11b est non-conjugé

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: M1-70-15

Détail du produit anti-CD11b anticorps

Réactivité croisée (Details): Species reactivity (expected):Human, Rabbit.
Species reactivity (tested):Mouse.

Purification: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

niveau d'endotoxine: Low Endotoxin

Immunogène: T cell enriched splenocytes from B10 mice. Spleen cells from immunised DA rats were fused with cells of the NS1/1.Ag4.1 mouse myeloma cell line.

Isotype: IgG2b

Information d'application

Indications d'application: Immunohistochemistry on frozen sections. Immunohistochemistry on paraffin sections. Immunoprecipitation. Flow Cytometry: 1/50 - 1/100 (routinely tested in flow cytometry on mouse peritoneal macrophages). Functional Assays: Clone M1/70.15 has been reported to block iC3b binding to its receptor.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Endotoxin Level < 0.01EU/ug

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Détails sur CD11b

Antigène: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Autre désignation: CD11b

Sujet: The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages.Synonyms: CD11 antigen-like family member B, CR-3 alpha chain, CR3A, Cell surface glycoprotein MAC-1 subunit alpha, ITGAM, Integrin alpha-M, Leukocyte adhesion receptor MO1, MAC1, Neutrophil adherence receptor

ID gène: 10090

UniProt: P05555

Pathways: Apoptose, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation