ATP6V0D2 anticorps (Isoform 1)

N° du produit ABIN3197488

Détail du produit anti-ATP6V0D2 anticorps

Antigène: ATP6V0D2 (ATPase, H+ Transporting, Lysosomal 38kDa, V0 Subunit D2 (ATP6V0D2))

Épitope: Isoform 1

Reactivité: Tomato, Arabidopsis thaliana, Zea mays, Pteris vittata (Chinese ladder brake), Oryza sativa, Lilium longiflorum (Trumpet lily), Chlamydomonas reinhardtii, Orge

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ATP6V0D2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Réactivité croisée (Details): No cross-reactivity with: mangrove plants,

Attributs du produit: Expected / apparent Molecular Weight of the Antigene: 26 / 31 kDa (Arabidopsis thaliana)

Purification: serum

Immunogène: KLH-conjugated synthetic peptide chosen from subunit E of plant V-ATPase including Arabidopsis thaliana At4g11150. Peptide is conserved in vacuolar H+-ATPase subunit E, isoform 1 to 3 (VHA-E1).

Information d'application

Indications d'application: Recommended Dilution: 1 : 2 000 - 1 : 5000 with alkaline phosphatase (WB), 1 : 50 (IHC).
Cellular [compartment marker] of tonoplast membrane

Commentaires:

V-ATPase is very sensitive for the redox of the SDS buffer. We recommend using at least 50-100 mM DTT freshly prepared before handling the sample.Immunostaining protocol using V-ATPase antibodies can be found here.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 10 µL of sterile water.

Conseil sur la manipulation: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Stock: -20 °C

Références pour anticorps anti-ATP6V0D2 (ABIN3197488)

Bigeard, Rayapuram, Bonhomme, Hirt, Pflieger: "Proteomic and phosphoproteomic analyses of chromatin-associated proteins from Arabidopsis thaliana." dans: Proteomics, Vol. 14, Issue 19, pp. 2141-55, (2014) (PubMed).

Schnell, Han, Miki, Johnson: "Soybean peroxidase propeptides are functional signal peptides and increase the yield of a foreign protein." dans: Plant cell reports, (2010) (PubMed).

Hinton, Sennoune, Bond, Fang, Reuveni, Sahagian, Jay, Martinez-Zaguilan, Forgac: "Function of a subunit isoforms of the V-ATPase in pH homeostasis and in vitro invasion of MDA-MB231 human breast cancer cells." dans: The Journal of biological chemistry, Vol. 284, Issue 24, pp. 16400-8, (2009) (PubMed).

Détails sur ATP6V0D2

Antigène: ATP6V0D2 (ATPase, H+ Transporting, Lysosomal 38kDa, V0 Subunit D2 (ATP6V0D2))

Autre désignation: V-ATPase (ATP6V0D2 Produits)

Synonymes: anticorps ATP6D2, anticorps VMA6, anticorps AI324824, anticorps V-ATPase, anticorps 1620401A02Rik, anticorps ATPase H+ transporting V0 subunit d2, anticorps ATPase, H+ transporting, lysosomal V0 subunit D2, anticorps ATPase H+ transporting V0 subunit D2, anticorps ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d2 L homeolog, anticorps ATP6V0D2, anticorps Atp6v0d2, anticorps atp6v0d2.L

Sujet: AGI Code: At4g11150
Plant vacuole V-ATPase is responsible for energization of transport of ions and metabolites, and acts as well 'house-keeping' and as a stress response enzyme. V-ATPase is a multi-subunit enzyme composed of a membrane sector and a cytosolic catalytic sector. It is related to the FoF1 ATP synthase. Alternative protein names: Vacuolar proton pump subunit E, Protein EMBRYO DEFECTIVE 2448

Poids moléculaire: expected: 26 kDa, apparent: 31 kDa (Arabidopsis thaliana)

UniProt: Q39258

Pathways: Transition Metal Ion Homeostasis, Proton Transport