MDM4-binding Protein anticorps (pSer367)

N° du produit ABIN3201009

Cet anticorps Souris Monoclonal détecte spécifiquement MDM4-binding Protein dans WB, ELISA et IP. Il présente une réactivité avec des échantillons de Humain et Souris. Il a été cité dans 2+ publications.

Aperçu rapide pour MDM4-binding Protein anticorps (pSer367) (ABIN3201009)

Antigène: MDM4-binding Protein (MDM4) (Mdm4-binding Protein (MDM4))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MDM4-binding Protein est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Clone: #15

Détail du produit anti-MDM4-binding Protein anticorps

Épitope: pSer367

Attributs du produit: Product: Mouse monoclonal antibody (clone #15) specific to the MdmX protein phosphorylated at Ser367, Human and mouse MdmX proteins phosphorylated at Ser367

Purification: Purified

Immunogène: A synthetic peptide corresponding to a sequence of human Mdmx protein surrounding pSer367

Isotype: IgG2b kappa

Information d'application

Indications d'application: 1. Western blotting: ~1 g/mL
2. Immunoprecipitation
3. ELISA
4. Indirect immuno-staining

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS with 50 % Glycerol

Agent conservateur: Azide free

Stock: -20 °C/-80 °C

Stockage commentaire: -20 C (For long term storage: -70 C)

Références pour anticorps anti-MDM4-binding Protein (ABIN3201009)

Chen, Gilkes, Pan, Lane, Chen: "ATM and Chk2-dependent phosphorylation of MDMX contribute to p53 activation after DNA damage." dans: The EMBO journal, Vol. 24, Issue 19, pp. 3411-22, (2005) (PubMed).

Okamoto, Kashima, Pereg, Ishida, Yamazaki, Nota, Teunisse, Migliorini, Kitabayashi, Marine, Prives, Shiloh, Jochemsen, Taya: "DNA damage-induced phosphorylation of MdmX at serine 367 activates p53 by targeting MdmX for Mdm2-dependent degradation." dans: Molecular and cellular biology, Vol. 25, Issue 21, pp. 9608-20, (2005) (PubMed).

Détails sur MDM4-binding Protein

Antigène: MDM4-binding Protein (MDM4) (Mdm4-binding Protein (MDM4))

Autre désignation: Mdmx/Hdmx

Sujet: MdmX (synonyms: Mdm4, HdmX) inhibits p53-and p73-dependent cell cycle arrest and apoptosis by binding to the transcription activation domains of these proteins. MdmX consists of 490 amino acids with the molecular weight of 54,864 and contains a RIING-finger domain and a nuclear transport signal. It is known that the protein migrates aberrantly in SDS-PAGE at the position of an 80- kDa protein. MdmX is phosphorylated at Ser367 by Chk2 kinase downstream of ATM in response to DNA damage, and as a result, it binds to14-3-3 and is transported into nucleus where it is degraded by Mdm2. This process activates the p53 functions.

UniProt: O15151

Pathways: Cycle Cellulaire