IL15RA anticorps (Extracellular Domain)

N° du produit ABIN327397

Détail du produit anti-IL15RA anticorps

Antigène: IL15RA (Interleukin 15 Receptor, alpha (IL15RA))

Épitope: Extracellular Domain

Reactivité: Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp IL15RA est non-conjugé

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Neutralization (Neut), Functional Studies (Func)

Specificité: Recognizes mouse IL-15Ra.

Purification: Immunoaffinity purified

Immunogène: NS0-derived rmIL-15Ra extracellular domain.

Type of Immunogen: Recombinant protein

Isotype: IgG

Information d'application

Indications d'application: Approved: ELISA, Flo, Func, Neut, WB

Usage: Neutralization of bioactivity, Direct ELISA, Western Blot, Flow Cytometry. Neutralization of Mouse IL-15 R Bioactivity: The exact concentration of antibody required to neutralize the mouse cell surface IL-15 R mediated bioactivity is dependent on the cytokine concentration, cell type, growth conditions and the type of activity studied. The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cell surface IL-15 R mediated IL-15 response on a responsive cell line, at a specific IL-15 concentration. As shown in Figures 1 and 2 on the next page, the ND50 for this lot of anti-mouse IL-15 R antibody was determined to be approximately 0.3-1.5 μg/mL in the presence of 0.8 ng/mL of human IL-15, using the CTLL-2 cell proliferation assay. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse IL-15 R. The detection limit for rmIL-15 R is approximately 0.1 ng/well. In this format, this antibody shows approximately 5 % cross-reactivity with rhIL-15 R and less than 1 % cross-reactivity with rhIL-2 R, rmIL-2 R and rmIL-2 R. Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect mouse IL-15 R. The detection limit for rmIL-15 R is approximately 5 ng/lane under non-reducing and reducing conditions. Flow Cytometry: This antibody can be used at 3-10 μg/mL/1 x10⁶ with an appropriate secondary antibody for indirect immunofluorescence staining of cells by flow cytometry.

Commentaires:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitution: Reconstitute, sterile PBS. If 1 mL of PBS is used, the antibody concentration will be 100 μg/mL.

Concentration: Lot specific

Buffer: Lyophilized from PBS, 5 % trehalose. Reconstitution: Reconstitute, sterile PBS. If 1 mL of PBS is used, the antibody concentration will be 100 μg/mL.

Détails sur IL15RA

Antigène: IL15RA (Interleukin 15 Receptor, alpha (IL15RA))

Autre désignation: IL15RA (IL15RA Produits)

Synonymes: anticorps IL15RA, anticorps CD215, anticorps AA690181, anticorps IL-15RA, anticorps interleukin 15 receptor subunit alpha, anticorps interleukin 15 receptor, alpha chain, anticorps IL15RA, anticorps Il15ra

Sujet: Name/Gene ID: IL15RA
Family: Interleukin

Synonyms: IL15RA, CD215 antigen, IL-15RA, IL-15 receptor subunit alpha, IL-15R-alpha, Interleukin 15 receptor, alpha, CD215

ID gène: 3601

UniProt: Q13261

Pathways: Signalistation JAK/STAT