CD14 anticorps (AA 9-13, AA 39-44)

N° du produit ABIN343167

Détail du produit anti-CD14 anticorps

Antigène: CD14

Épitope: AA 9-13, AA 39-44

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD14 est non-conjugé

Application: Flow Cytometry (FACS), ELISA, Functional Studies (Func), Blocking Peptide (BP)

Specificité: Soluble and membrane bound human CD14. The antibody blocks LPS binding to membrane bound CD14

Réactivité croisée: Corynebacterium diphtheriae, Boeuf (Vache), Cheval

Réactivité croisée (Details): horse and bovine CD14,inhibits binding of LPS to horse and bovine CD14. Binding titre at horse CD14 1:100 and inhibition titre 1:10.

Purification: Protein A affinity column

Immunogène: Monocytes of different species and immunoaffinity purified soluble human CD14

Clone: BiG10

Isotype: IgG1

Information d'application

Indications d'application: total inhibition of LPS-binding to CD14: 100 μg/mL. Binding titre at human CD14 transfected CHO-cells: more than 10.000.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute with bidistilled water

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4

Agent conservateur: Without preservative

Stock: -20 °C,-80 °C

Références pour anticorps anti-CD14 (ABIN343167)

Hackett, Flaminio, Fortier: "Analysis of CD14 Expression Levels in Putative Mesenchymal Progenitor Cells Isolated from Equine Bone Marrow." dans: Stem cells and development, (2010) (PubMed).

Mérant, Breathnach, Kohler, Rashid, Van Meter, Horohov: "Young foal and adult horse monocyte-derived dendritic cells differ by their degree of phenotypic maturity." dans: Veterinary immunology and immunopathology, Vol. 131, Issue 1-2, pp. 1-8, (2009) (PubMed).

Stelter, Pfister, Bernheiden, Jack, Bufler, Engelmann, Schuett: "The myeloid differentiation antigen CD14 is N- and O-glycosylated. Contribution of N-linked glycosylation to different soluble CD14 isoforms." dans: European journal of biochemistry / FEBS, Vol. 236, Issue 2, pp. 457-64, (1996) (PubMed).

Détails sur CD14

Antigène: CD14

Autre désignation: CD14 (CD14 Produits)

Synonymes: anticorps CD14 molecule, anticorps CD14 antigen, anticorps CD14, anticorps Cd14

Sujet: Background: biG 10 represents an excellent marker for CD14. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophages like cell types: monocytes, macrophages, weekly at surface of neutrophiles like Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 Molecule has been reported to be a receptor for endotoxin. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for LPS-LBP (lipopolysaccharide binding protein)-complexes.

Poids moléculaire: ~50kDa

ID gène: 929

NCBI Accession: NP_000582

UniProt: P08571

Pathways: Signalisation TLR, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades