CD14 anticorps (AA 39-41, AA 43-44)

N° du produit ABIN343169

Il existe 3+ publications pour ce produit. L’anticorps anti-CD14 Monoclonal Souris est utilisé pour la détection de CD14 dans des échantillons de Humain. Il a été validé pour FACS, WB, IF et ELISA.

Aperçu rapide pour CD14 anticorps (AA 39-41, AA 43-44) (ABIN343169)

Antigène: CD14

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD14 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), ELISA

Clone: BiG14

Détail du produit anti-CD14 anticorps

Épitope: AA 39-41, AA 43-44

Specificité: Soluble and membrane bound human CD14 The antibody blocks LPS binding to membrane bound CD14

Réactivité croisée: Corynebacterium diphtheriae, Boeuf (Vache), Chien, Cobaye, Cheval, Porc

Réactivité croisée (Details): cattle++, swine ++, dog+ horse+, guinea pig +,Inhibits binding of LPS to CD14 of swine++, dog+, cattle +, dog+, more than titer 10.000.

Purification: Protein A affinity column

Immunogène: Monocytes of different species and immunoaffinity purified soluble human CD14

Isotype: IgG1

Information d'application

Indications d'application: binding titre at human CD14 transfected CHO-cells: 1:50.000

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute with bidistilled water

Concentration: 1 mg/mL

Buffer: PBS, pH 7.6

Agent conservateur: Without preservative

Stock: -20 °C,-80 °C

Références pour anticorps anti-CD14 (ABIN343169)

Knobloch, Feldmann, Wahl, Jungck, Behr, Stoelben, Koch: "Endothelin receptor antagonists attenuate the inflammatory response of human pulmonary vascular smooth muscle cells to bacterial endotoxin." dans: The Journal of pharmacology and experimental therapeutics, (2013) (PubMed).

Bache, Spreitzer, Becker, Loeschner, Rosskopf, Hanschmann, Schwanig, Schneider, Lieb, Montag: "Bordetella Pertussis Toxin does not induce the release of pro-inflammatory cytokines in human whole blood." dans: Medical microbiology and immunology, Vol. 201, Issue 3, pp. 327-35, (2012) (PubMed).

Schromm, Reiling, Howe, Wiesmueller, Roessle, Brandenburg: "Influence of serum on the immune recognition of a synthetic lipopeptide mimetic of the 19-kDa lipoprotein from Mycobacterium tuberculosis." dans: Innate immunity, (2009) (PubMed).

Détails sur CD14

Antigène: CD14

Autre désignation: CD14

Sujet: Background: biG 14 represents an excellent marker for CD14. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophages like cell types: monocytes, macrophages, weekly at surface of neutrophiles like Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 Molecule has been reported to be a receptor for endotoxin. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for LPS-LBP (lipopolysaccharide binding protein)-complexes.

Poids moléculaire: ~50kDa

ID gène: 929

NCBI Accession: NP_000582

UniProt: P08571

Pathways: Signalisation TLR, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades