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Keratin 5/8 anticorps (AA 353-367) (Biotin)

Cet anticorps Souris Monoclonal détecte spécifiquement Keratin 5/8 dans FACS, IHC, IHC (p), IHC (fro) et ELISA. Il présente une réactivité envers Humain.
N° du produit ABIN344035

Aperçu rapide pour Keratin 5/8 anticorps (AA 353-367) (Biotin) (ABIN344035)

Antigène

Keratin 5/8 (KRT5/8)

Reactivité

  • 23
  • 17
  • 16
  • 14
  • 14
  • 14
  • 13
  • 11
  • 10
  • 3
  • 2
  • 2
Humain

Hôte

  • 23
Souris

Clonalité

  • 23
Monoclonal

Conjugué

  • 10
  • 3
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp Keratin 5/8 est conjugé à/à la Biotin

Application

  • 17
  • 15
  • 13
  • 13
  • 8
  • 8
  • 5
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA
  • Épitope

    AA 353-367

    Specificité

    Reactivities on Cultured MCF-7, RT 112, HT-29, Detroit 562, RPMI 2650. Cell lines (tested so far) SSC-12, bovine BMGE+H, BMGE-H, MDBK. Represents an excellent marker for distinguishing carcinomas from all non-epithelial tumors. Specifically reacts with cytokeratins 5 and 8 present in nearly all epithelia. Polypeptide reacting: Mr 52500, Mr 58000 cytokeratins (type II cytokeratins nos. 5+8) of human epithelial cells. Epitope has been mapped to aa353-367 on alpha helical rod domain. Species cross-reactivity: Human, bovine, murine, amphibia, porcine, hydra.

    Purification

    Protein A purified

    Immunogène

    Human cytokeratin 8, purified from SDS PAGE.

    Type of Immunogen: Purified protein

    Isotype

    IgG1
  • Indications d'application

    Approved: ELISA, Flo, IHC, IHC-Fr, IHC-P

    Usage: Suitable for use in ELISA, FACS and Immunohistochemistry (frozen and paraffin-embedded tissue and cytological material, Pretreatment With paraffin embedded sections, protease pretreatment is required prior to antibody application). Immunohistochemistry: 1:10-1:20, Incubation Time 1hr at RT, extended with paraffin sections (overnight at 2-8?C). The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

    Commentaires

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Concentration

    Lot specific
  • Antigène

    Keratin 5/8 (KRT5/8)

    Autre désignation

    Cytokeratin 5+8

    Sujet

    This antibody detects: Cytokeratin 5+8
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