POGZ anticorps (N-Term)

N° du produit ABIN964888

Détail du produit anti-POGZ anticorps

Antigène: POGZ (Pogo Transposable Element with ZNF Domain (POGZ))

Épitope: N-Term

Reactivité: Humain, Rat, Chien, Boeuf (Vache), Poulet, Chimpanzé, Macaque, Babouin olive, Opossum

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp POGZ est non-conjugé

Application: Western Blotting (WB), ELISA

Specificité: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography.  This antibody is known to react with mouse Pogz protein.  A BLAST analysis was used to suggest cross-reactivity with Pogz from human, dog, short-tailed opossum, cattle, rat, chimpanzee, macaque, olive baboon, and chicken sources based on 100% homology with the immunizing sequence.    Reactivity with Pogz from other sources has not been determined.

Réactivité croisée: Chien, Opossum (Didelphimorphia), Mouton, Rat (Rattus), Chimpanzé, Macaque, Babouin olive, Poulet

Attributs du produit: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Pogz is a zinc-finger protein containing at least 8 C2H2 zinc fingers, a CENP-B (centromere protein-B) domain, and a DDE domain found in the bacterial transposase/retroviral integrase family. Members of this family of integrases share structural homologies and show similar catalytic activity to the RAG1 and RAG2 proteins that initiate V(D)J recombination in lymphoid progenitors. Mouse Pogz has a human homolog with a base pair similarity of 90% and amino acid similarity of 93% for the full length protein. In humans at least 3 variants have been predicted and they are all similar at the N-terminus. A number of expressed sequence tags (ESTs) cloned from murine undifferentiated ES cells and Lin-/c-Kit+/Sca-1+ hematopoietic stem cell cDNA libraries correspond to the Pogz gene, further underlining an important function for this gene in stem cells. In mice, deletion of the gene in all tissues early in embryogenesis has been shown to be lethal.

Purification: affinity purified

Stérilité: Sterile filtered

Immunogène: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the N-terminus of mouse Pogz protein.
Immunogen Type: Peptide

Isotype: IgG

Information d'application

Indications d'application: This affinity purified antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 155 kDa in size corresponding to Pogz by western blotting in the appropriate cell lysate or extract.

Commentaires:

Gene Name: POGZ

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.6 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is three (3) months from date of opening.

Date de péremption: 3 months

Détails sur POGZ

Antigène: POGZ (Pogo Transposable Element with ZNF Domain (POGZ))

Autre désignation: Pogz (POGZ Produits)

Synonymes: anticorps POGZ, anticorps ZNF280E, anticorps ZNF635, anticorps ZNF635m, anticorps 9530006B08Rik, anticorps AU015913, anticorps AU044539, anticorps pogo transposable element with ZNF domain, anticorps pogo transposable element derived with ZNF domain, anticorps POGZ, anticorps pogz, anticorps Pogz

Sujet: This antibody is designed, produced, and is suitable for Cancer, Immunology and Nuclear Signaling research. Pogz is a zinc-finger protein containing at least 8 C2H2 zinc fingers, a CENP-B (centromere protein-B) domain, and a DDE domain found in the bacterial transposase/retroviral integrase family. Members of this family of integrases share structural homologies and show similar catalytic activity to the RAG1 and RAG2 proteins that initiate V(D)J recombination in lymphoid progenitors.  Mouse Pogz has a human homolog with a base pair similarity of 90% and amino acid similarity of 93% for the full length protein.  In humans at least 3 variants have been predicted and they are all similar at the N-terminus.   A number of expressed sequence tags (ESTs) cloned from murine undifferentiated ES cells and Lin-/c-Kit+/Sca-1+ hematopoietic stem cell cDNA libraries correspond to the Pogz gene, further underlining an important function for this gene in stem cells.  In mice, deletion of the gene in all tissues early in embryogenesis has been shown to be lethal.
Synonyms: pogo transposable element with ZNF domain antibody, putative protein product of Nbla00003 antibody, SUHW5 antibody, suppressor of hairy wing homolog antibody, zinc finger protein 280E antibody

ID gène: 229584

UniProt: Q8BZH4