STAT5A anticorps (pTyr694)

N° du produit ABIN349611

N° du produit (Fournisseur): 200-301-a45

Cet anticorps Souris Monoclonal détecte spécifiquement STAT5A dans WB, IHC, ELISA, IF, FM et MA. Il présente une réactivité envers Humain et Souris.

Aperçu rapide pour STAT5A anticorps (pTyr694) (ABIN349611)

Antigène: STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp STAT5A est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM), Multiplex Assay (MA)

Clone: 5F6-F1

Détail du produit anti-STAT5A anticorps

Épitope: pTyr694

N° du produit (Fournisseur): 200-301-a45

Fournisseur: Rockland

Fonction: Stat5 phospho Y694 Antibody

Réactivité croisée (Details): This antibody is specific for mouse Stat5a protein phosphorylated at Y694.

Attributs du produit: Synonyms: mouse anti-STAT5 pY694 Antibody, MGF antibody, Signal Transducer and Activator of Transcription 5A antibody, STAT 5 antibody

Purification: Phospho STAT5 pY694 was purified from concentrated tissue culture supernate by Protein A chromatography.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Anti-STAT5 phospho Y694 monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y694 of mouse STAT5a protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 20-40 μg/mL

Application Note: Phospho STAT5 pY694 monoclonal antibody has been tested by ELISA, western blot, immunohistochemistry, and immunofluorescence.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 91 kDa in size corresponding to phosphorylated Stat5a protein by western blotting in the appropriate cell lysate or extract.  This phospho-specific monoclonal antibody reacts with mouse Stat5a pY694 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:20,000

IF Microscopy Dilution: 1:50-1:1000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store STAT5 phospho Y694 Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Phospho STAT5 antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur STAT5A

Antigène: STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))

Autre désignation: Stat5a

Sujet: Background: Signal transducer and activator of transcription 5 (Stat5) belongs to a family of cytoplasmic transcription factors that can be activated (phosphorylated) by a cell surface receptor. Phosphorylation at Tyr694 is obligatory for Stat5 activation. Stat5 has two isoforms, Stat5α and Stat5ß. Aberrant Stat5 activation has been implicated in the pathogenesis of chronic myelogenous leukemia, prostate and breast cancer and tumor metastasis. Stat5 is localized in the cytoplasm and upon phosphorylation at Y694 is translocated to the nucleus. Ideal for Cancer, Chromatin & Nuclear Signaling and Signal Transduction research.

ID gène: 20850, 6755672

UniProt: P42230

Pathways: Signalistation JAK/STAT, Signalisation RTK, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Activated T Cell Proliferation