ERCC3 anticorps (AA 242-261)

N° du produit ABIN351537

Détail du produit anti-ERCC3 anticorps

Antigène: ERCC3 (DNA Repair Protein Complementing XP-B Cells (ERCC3))

Épitope: AA 242-261

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ERCC3 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Recognizes an epitope located between aa242-261 of ERCC-3 (largest subunit of TFIIH).

Purification: Protein G purified

Immunogène: Synthetic peptide corresponding to aa242-261 of human ERCC-3.

Type of Immunogen: Synthetic peptide

Clone: 3G4

Isotype: IgG2b

Information d'application

Indications d'application: Approved: ELISA, IHC, IHC-P (5 - 10 μg/mL), IP, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5-10 μg/mL. Immunoprecipitation: Immunoprecipitates transcriptionally active TFIIH in a reconstituted assay.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-HCl,  pH 7.5. Sourced from ascites.

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur ERCC3

Antigène: ERCC3 (DNA Repair Protein Complementing XP-B Cells (ERCC3))

Autre désignation: ERCC3 / XPB (ERCC3 Produits)

Synonymes: anticorps BTF2, anticorps GTF2H, anticorps RAD25, anticorps TFIIH, anticorps XPB, anticorps BTF2 p89, anticorps Ercc-3, anticorps wu:fc25f08, anticorps ERCC excision repair 3, TFIIH core complex helicase subunit, anticorps excision repair cross-complementing rodent repair deficiency, complementation group 3, anticorps excision repair cross-complementation group 3, anticorps ERCC3, anticorps Ercc3, anticorps ercc3

Sujet: Name/Gene ID: ERCC3

Synonyms: ERCC3, BTF2, GTF2H, RAD25, TFIIH p89, TFIIH, XPB, BTF2 p89, XPBC, TFIIH 89 kDa subunit

ID gène: 2071

UniProt: P19447

Pathways: Réparation de l'ADN