ERCC3 anticorps (N-Term)

N° du produit ABIN6261590

Détail du produit anti-ERCC3 anticorps

Antigène: ERCC3 (DNA Repair Protein Complementing XP-B Cells (ERCC3))

Épitope: N-Term

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ERCC3 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Specificité: ERCC3 Antibody detects endogenous levels of total ERCC3.

Homologie: Pig,Zebrafish,Bovine,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogène: A synthesized peptide derived from human ERCC3, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Information d'application

Indications d'application: WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20 °C. Stable for 12 months from date of receipt.

Date de péremption: 12 months

Détails sur ERCC3

Antigène: ERCC3 (DNA Repair Protein Complementing XP-B Cells (ERCC3))

Autre désignation: ERCC3 (ERCC3 Produits)

Synonymes: anticorps BTF2, anticorps GTF2H, anticorps RAD25, anticorps TFIIH, anticorps XPB, anticorps BTF2 p89, anticorps Ercc-3, anticorps wu:fc25f08, anticorps ERCC excision repair 3, TFIIH core complex helicase subunit, anticorps excision repair cross-complementing rodent repair deficiency, complementation group 3, anticorps excision repair cross-complementation group 3, anticorps ERCC3, anticorps Ercc3, anticorps ercc3

Sujet:

Description: ATP-dependent 3'-5' DNA helicase, component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The ATPase activity of XPB/ERCC3, but not its helicase activity, is required for DNA opening. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. The ATP-dependent helicase activity of XPB/ERCC3 is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.

Gene: ERCC3

Poids moléculaire: 89kDa

ID gène: 2071

UniProt: P19447

Pathways: Réparation de l'ADN