DKK4 anticorps (N-Term)

N° du produit ABIN357073

Cet anticorps Lapin Polyclonal détecte spécifiquement DKK4 dans WB, IHC (p) et EIA. Il présente une réactivité envers Humain.

Aperçu rapide pour DKK4 anticorps (N-Term) (ABIN357073)

Antigène: DKK4 (Dickkopf Homolog 4 (Xenopus Laevis) (DKK4))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DKK4 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-DKK4 anticorps

Épitope: AA 7-36, N-Term

Séquence: DKK4

Specificité: This antibody detects DKK4 at N-term.

Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogène: KLH conjugated synthetic peptide between 7~36 amino acids from the N-terminal region of Human DKK4

Isotype: Ig Fraction

Information d'application

Indications d'application: ELISA 1: 1,000. Western blot 1: 100 - 1: 500. Immunohistochemistry 1: 50 - 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Détails sur DKK4

Antigène: DKK4 (Dickkopf Homolog 4 (Xenopus Laevis) (DKK4))

Autre désignation: Dickkopf-4 (DKK4)

Sujet: DKK4, like DKK1, DKK2, and DKK3, possesses an N-terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region and contain 10 cysteine residues each. The second cysteine region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis detected no expression of DKK4, but RT-PCR analysis detected DKK4 expression in cerebellum, T-cell, esophagus, and lung cDNA libraries. DKK4 blocks Xenopus Wnt8, Wnt3a, and Wnt2b, but not Dsh or Fz8, induction of a secondary axis in frog embryos, indicating that DKKs antagonize WNT function upstream of WNT receptors.Synonyms: Dickkopf-related protein 4, Dkk-4

Poids moléculaire: 24875 Da

ID gène: 27121, 9606

UniProt: Q9UBT3

Pathways: Signalisation WNT