Myeloid/lymphoid Or Mixed-Lineage Leukemia (Trithorax Homolog, Drosophila), Translocated To, 3 (MLLT3) (C-Term) anticorps

Détails pour le produit réf. ABIN358673, Fournisseur: Connectez-vous pour afficher
Antigène
  • fc39c11
  • wu:fc39c11
  • zgc:110210
  • AF9
  • YEATS3
  • 2210011H10Rik
  • 2610012I03Rik
  • 3830408D16Rik
  • Af9
  • D4Ertd321e
  • Af-9
  • MLLT3, super elongation complex subunit
  • myeloid/lymphoid or mixed-lineage leukemia; translocated to, 3
  • mllt3
  • MLLT3
  • Mllt3
Épitope
C-Term
28
11
8
7
7
6
4
4
3
3
3
2
1
1
1
1
1
1
1
Reactivité
Humain
88
20
10
7
7
6
6
4
3
2
2
1
Hôte
Lapin
85
3
Clonalité
Polyclonal
Conjugué
Inconjugué
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
78
43
35
31
21
17
5
3
2
Options
Fournisseur
Connectez-vous pour afficher
N° du produit (Fournisseur)
Connectez-vous pour afficher
Immunogène This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human MLLT3.
Isotype Ig Fraction
Specificité This antibody reacts to AF9 (MLLT3).
Réactivité croisée (Details) Species reactivity (expected):Mouse.
Species reactivity (tested):Human.
Purification Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Antigène
Autre désignation MLLT3 / AF9 (MLLT3 Antibody Extrait)
Sujet The human AF9 gene is one of the most common fusion partner genes with the ALL1 gene at 11q23 (also called MLL), resulting in the t(9,11)(p22,q23). The AF9 gene is more than 100 kb, and 2 patient breakpoint cluster regions (BCRs) have been identified, BCR1 is within intron 4, previously called site A, whereas BCR2 or site B spans introns 7 and 8. Several different structural elements have been identified in AF9, including a colocalizing in vivo DNA topo II cleavage site and an in vitro DNase I hypersensitive (DNase 1 HS) site in intron 7 in BCR2. Reversibility experiments demonstrated a religation of the topo II cleavage sites. In addition, 2 scaffold associated regions (SARs) are located centromeric to the topo II and DNase I HS cleavage sites and border breakpoint regions in 2 leukemic cells lines: SAR1 is located in intron 4, whereas SAR2 encompasses parts of exons 5-7. The patient breakpoint regions of AF9 share the same structural elements as the MLL BCR. A DNA breakage and repair model for nonhomologous recombination between MLL and its partner genes, particularly AF9, has been proposed.Synonyms: ALL1 fused gene from chromosome 9 protein, Myeloid/lymphoid or mixed-lineage leukemia translocated to chromosome 3 protein, Protein AF-9, YEATS domain-containing protein 3, YEATS3
Poids moléculaire 63351 Da
ID gène 4300, 5874
UniProt P42568
Indications d'application ELISA: 1/1,000. Immunohistochemistry: 1/50 - 1/100. Western Blot: 1/100 - 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS, 0.09 % (W/V) Sodium Azide
Agent conservateur Sodium azide
Précaution d'utilisation This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Avoid repeated freezing and thawing.
Stock 4 °C/-20 °C
Stockage commentaire Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Images (Fournisseur)
 image for anti-Myeloid/lymphoid Or Mixed-Lineage Leukemia (Trithorax Homolog, Drosophila), Translocated To, 3 (MLLT3) (C-Term) antibody (ABIN358673) anti-Myeloid/lymphoid Or Mixed-Lineage Leukemia (Trithorax Homolog, Drosophila), Translocated To, 3 (MLLT3) (C-Term) antibody
 image for anti-Myeloid/lymphoid Or Mixed-Lineage Leukemia (Trithorax Homolog, Drosophila), Translocated To, 3 (MLLT3) (C-Term) antibody (ABIN358673) anti-Myeloid/lymphoid Or Mixed-Lineage Leukemia (Trithorax Homolog, Drosophila), Translocated To, 3 (MLLT3) (C-Term) antibody (Image 2)
Produit citée dans: Benedikt, Baltruschat, Scholz, Bursen, Arrey, Meyer, Varagnolo, Müller, Karas, Dingermann, Marschalek: "The leukemogenic AF4-MLL fusion protein causes P-TEFb kinase activation and altered epigenetic signatures." dans: Leukemia, Vol. 25, Issue 1, pp. 135-44, 2011 (PubMed).

Background publications Bitoun, Oliver, Davies: "The mixed-lineage leukemia fusion partner AF4 stimulates RNA polymerase II transcriptional elongation and mediates coordinated chromatin remodeling." dans: Human molecular genetics, Vol. 16, Issue 1, pp. 92-106, 2007 (PubMed).

Avez-vous cherché autre chose?