DARPP32 anticorps (N-Term)

N° du produit ABIN361423

Détail du produit anti-DARPP32 anticorps

Antigène: DARPP32 (PPP1R1B) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B))

Épitope: N-Term

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DARPP32 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificité: Specific for the ~32k DARPP-32 protein.

Réactivité croisée: Rat (Rattus)

Homologie: bovine, canine, chicken, human, mouse, non-human primate, Xenopus

Purification: Antigen Affinity Purified from Pooled Serum

Immunogène: Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH

Information d'application

Indications d'application: Recommended Dilution: WB: 1:1000 IHC: 1:250 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Stock: -20 °C

Références pour anticorps anti-DARPP32 (ABIN361423)

Gomez, Midde, Mactutus, Booze, Zhu: "Environmental enrichment alters nicotine-mediated locomotor sensitization and phosphorylation of DARPP-32 and CREB in rat prefrontal cortex." dans: PLoS ONE, Vol. 7, Issue 8, pp. e44149, (2013) (PubMed).

Détails sur DARPP32

Antigène: DARPP32 (PPP1R1B) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B))

Autre désignation: PPP1R1B (PPP1R1B Produits)

Synonymes: anticorps darpp32, anticorps darpp-32, anticorps Darpp-32, anticorps Darpp32, anticorps DARPP-32, anticorps DARPP32, anticorps AU040756, anticorps protein phosphatase 1 regulatory inhibitor subunit 1B, anticorps protein phosphatase 1, regulatory (inhibitor) subunit 1B, anticorps protein phosphatase 1 regulatory inhibitor subunit 1B S homeolog, anticorps ppp1r1b, anticorps PPP1R1B, anticorps Ppp1r1b, anticorps ppp1r1b.S

Sujet: DARPP-32 is a dopamine (DA) and cAMP-regulated ~32k phosphoprotein that is associated with dopaminoceptive neurons (Fienberg et al., 1998). The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA (Bibb et al., 1999). Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac® (Maldve et al., 2002, Lindskog et al., 2002, Svenningsson et al., 2002). Anti-DARPP-32 Western blot of a rat hippocampal lysate showing specific immunolabeling of the ~32k DARPP protein.

Poids moléculaire: '32 kDa

ID gène: 360616

UniProt: Q6J4I0