Parkin anticorps (pSer378)

N° du produit ABIN361515

Cet anticorps anti-Parkin est un anticorps Lapin Polyclonal détectant Parkin dans WB. Adapté pour Humain.

Aperçu rapide pour Parkin anticorps (pSer378) (ABIN361515)

Antigène: Parkin (PARK2) (Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Parkin est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-Parkin anticorps

Épitope: pSer378

Specificité: Specific for the ~52k parkin protein phosphorylated at Ser378. Immunolabeling of the parkin band is absent in parkin S378 mutants.

Réactivité croisée: Humain

Purification: Antigen Affinity Purified

Immunogène: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser378 conjugated to KLH

Information d'application

Indications d'application: Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Stock: -20 °C

Détails sur Parkin

Antigène: Parkin (PARK2) (Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2))

Autre désignation: PARK2

Sujet: Parkin is an E3 ligase in the ubiquitin-proteasome system. Hereditary Parkinson’s disease is most commonly caused by mutations in the parkin gene and is characterized by the progressive loss of dopaminergic neurons and the presence of Lewy bodies in the substania nigra (Jenner et al.,1992). Recent evidence suggests that phosphorylation of parkin at Ser378 may have an important regulatory role on its E3 ubiquitin ligase activity (Yamamoto et al., 2005). Anti-Phospho-Ser378 Parkin Western blot of HEK293 cells transfected with Parkin WT (Phospho) and Parkin S378 mutant (non-phospho) showing the phospho-specific immunolabeling of the ~ 52 k parkin protein. The immunolabeling is absent in the parkin S378 mutant.

Poids moléculaire: '52 kDa

ID gène: 5071

UniProt: O60260

Pathways: Autophagy, Ubiquitin Proteasome Pathway