EIF2AK2 anticorps (pThr451)

N° du produit ABIN362374

Cité dans 1 publication. Cet anticorps anti-EIF2AK2 Polyclonal Lapin (ABIN362374) détecte spécifiquement EIF2AK2 dans IHC. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour EIF2AK2 anticorps (pThr451) (ABIN362374)

Antigène: EIF2AK2 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp EIF2AK2 est non-conjugé

Application: Immunohistochemistry (IHC)

Détail du produit anti-EIF2AK2 anticorps

Épitope: pThr451

Specificité: The antibody detects endogenous level of PKR only when phosphorylated at threonine 451.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogène: Peptide sequence around phosphorylation site of pThr451 (K-G-T (p) -L-R) derived from Human PKR. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Information d'application

Indications d'application: Immunohistochemistry: 1:50-1:100

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Références pour anticorps anti-EIF2AK2 (ABIN362374)

Laing, Wang, Briazova, Zhang, Wang, Zheng, Gow, Chen, Rajagopalan, Chen, Sun, Zhang: "Airborne particulate matter selectively activates endoplasmic reticulum stress response in the lung and liver tissues." dans: American journal of physiology. Cell physiology, Vol. 299, Issue 4, pp. C736-49, (2010) (PubMed).

Détails sur EIF2AK2

Antigène: EIF2AK2 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2))

Autre désignation: PKR

Sujet: Following activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection.

Poids moléculaire: 68 kDa

NCBI Accession: NP_001129

UniProt: P19525

Pathways: Réparation de l'ADN, ER-Nucleus Signaling, Hepatitis C