Leptin Receptor anticorps (AA 829-841)

N° du produit ABIN364407

Cet anticorps anti-Leptin Receptor est un anticorps Chèvre Polyclonal détectant Leptin Receptor dans WB, ELISA, IHC et IHC (p). Adapté pour Humain.

Aperçu rapide pour Leptin Receptor anticorps (AA 829-841) (ABIN364407)

Antigène: Leptin Receptor (LEPR)

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Leptin Receptor est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-Leptin Receptor anticorps

Épitope: AA 829-841

Marque: IHC-plus™

Specificité: Human LEPR / Leptin Receptor. This antibody is expected to recognise all three reported isoforms (NP_002294.2, NP_001003679.1, NP_001003680.1).

Homologie: Percent identity by BLAST analysis: Human, Gorilla (100%) Marmoset, Panda, Dog, Horse (92%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide C-TQDDIEKHQSDAG from an internal region of human LEPR / Leptin Receptor (NP_002294.2, NP_001003679.1, NP_001003680.1). Percent identity by BLAST analysis: Human, Gorilla (100%), Marmoset, Panda, Dog, Horse (92%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (4 μg/mL), Peptide-ELISA (1:16000), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 4 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur Leptin Receptor

Antigène: Leptin Receptor (LEPR)

Autre désignation: LEPR / Leptin Receptor

Sujet: Name/Gene ID: LEPR

Synonyms: LEPR, CD295 antigen, HuB219, LEP-R, OB receptor, LEPRD, OB-R, OBR, CD295, DB, Leptin receptor

ID gène: 3953

NCBI Accession: NP_002294, NP_001003679, NP_001003680

UniProt: P48357

Pathways: Signalistation JAK/STAT, AMPK Signaling, Feeding Behaviour