MAPK14 anticorps (pThr180, pTyr182)

N° du produit ABIN372694

Cet anticorps Lapin Polyclonal détecte spécifiquement MAPK14 dans WB et IHC (fro). Il présente une réactivité envers Humain, Souris, Rat, Chien, Poulet, Boeuf (Vache), Poisson zèbre (Danio rerio) et Non-Human Primate et a été mentionné dans 1 publication.

Aperçu rapide pour MAPK14 anticorps (pThr180, pTyr182) (ABIN372694)

Antigène: MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))

Reactivité: Humain, Souris, Rat, Chien, Poulet, Boeuf (Vache), Poisson zèbre (Danio rerio), Non-Human Primate

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MAPK14 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-MAPK14 anticorps

Épitope: pThr180, pTyr182

Specificité: This antibody recognizes the ~39k p38 MAPK protein phosphorylated at Thr180/Tyr182. Immunolabeling is blocked by preadsorption of the antibody with the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.

Réactivité croisée (Details): Species reactivity (expected):Bovine, Canine, Chicken, Mouse, Zebrafish and non-Human Primates.
Species reactivity (tested):Human.

Purification: Sequential Chromatography on phospho- and dephosphopeptide affinity columns.

Immunogène: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr180 and phospho-Tyr182 of rat p38 MAPK.

Isotype: IgG

Information d'application

Indications d'application: Western blot: 1/1000. Immunohistochemistry: 1/250-1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody undiluted (in aliquots) at-20 °C.

Références pour anticorps anti-MAPK14 (ABIN372694)

Chaudhuri, Ghate, Singh, Mandal: "Methyl gallate isolated from Spondias pinnata exhibits anticancer activity against human glioblastoma by induction of apoptosis and sustained extracellular signal-regulated kinase 1/2 activation." dans: Pharmacognosy magazine, Vol. 11, Issue 42, pp. 269-76, (2015) (PubMed).

Détails sur MAPK14

Antigène: MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))

Autre désignation: MAP Kinase p38 alpha / MAPK14

Sujet: The three Mitogen-Activated Protein Kinases (MAPKs) are evolutionarily conserved protein kinases that control a vast array of cellular processes. p38 MAPK is one of these kinases and it is activated by both inflammatory cytokines and by stress (Johnson and Lapadat, 2002, Shi and Gaestel, 2002). The p38 MAPK is thought to be particularly important in diseases like asthma and autoimmunity but it also plays important roles in the stress response of the nervous system (Philip and Armstead, 2003, Ying et al., 2002). Like the other MAPKs, p38 is activated by a dual specificity kinase that phosphorylates Thr180 and Tyr182 (Lin et al., 1995).Synonyms: CSBP, CSBP1, CSBP2, CSPB1, Cytokine suppressive anti-inflammatory drug-binding protein, MAX-interacting protein 2, MXI2, Mitogen-activated protein kinase 14, SAPK2A, p38 alpha

ID gène: 1432

NCBI Accession: NP_001306

UniProt: Q16539

Pathways: Signalisation MAPK, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Hepatitis C, Toll-Like Receptors Cascades, Autophagy, Thromboxane A2 Receptor Signaling, BCR Signaling, S100 Proteins