SMAD4 anticorps (C-Term)

N° du produit ABIN374104

Cet anticorps Chèvre Polyclonal détecte spécifiquement SMAD4 dans WB et EIA. Il présente une réactivité avec des échantillons de Humain, Souris, Rat, Boeuf (Vache), Chien et Porc.

Aperçu rapide pour SMAD4 anticorps (C-Term) (ABIN374104)

Antigène: SMAD4 (SMAD Family Member 4 (SMAD4))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien, Porc

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp SMAD4 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-SMAD4 anticorps

Épitope: C-Term

Séquence: C-HTMPIADPQP LD

Specificité: This antibody detects SMAD4 at C-term.

Réactivité croisée (Details): Species reactivity (expected):Human, Rat, Canine (Dog), Pig, Cow.
Species reactivity (tested):Mouse.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogène: Peptide from C-Terminus of the protein sequence according to NP_005350.1 Genename: SMAD4

Information d'application

Indications d'application: Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.5-2 μg/mL. Approx 60-65 kDa band seen in A431 cell lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur SMAD4

Antigène: SMAD4 (SMAD Family Member 4 (SMAD4))

Autre désignation: SMAD4

Sujet: SMAD4 (also known as Mothers against decapentaplegic homolog 4, Mothers against DPP homolog 4, deletion target in pancreatic carcinoma 4 and hSMAD4) is a common mediator of signal transduction by TGF-beta (transforming growth factor), but is also involved in cancer development and metastases as a tumor suppressor. SMAD4 promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. SMAD4 may form trimers with receptor-regulated SMAD (R-SMAD) and interacts with ATF2, COPS5, DACH1, MSG1, SKI and TRIM33. In the absence of ligand SMAD4 is found in the cytoplasm, but when complexed with R-SMAD, translocates to the nucleus. Defects in SMAD4 are a cause of pancreatic carcinoma and juvenile polyposis syndrome (JPS), a syndrome in which patients are at risk for developing gastrointestinal cancers.Synonyms: DPC4, Deletion target in pancreatic carcinoma 4, MAD homolog 4, MADH4, Mothers against DPP homolog 4, Mothers against decapentaplegic homolog 4, SMAD 4, SMAD family member 4, SMAD-4

ID gène: 4089

NCBI Accession: NP_005350

UniProt: Q13485

Pathways: Cycle Cellulaire, Chromatin Binding, Autophagy