KLF15 anticorps (N-Term)

N° du produit ABIN374226

Détail du produit anti-KLF15 anticorps

Antigène: KLF15 (Kruppel-Like Factor 15 (KLF15))

Épitope: N-Term

Reactivité: Humain, Souris, Rat, Porc

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp KLF15 est non-conjugé

Application: Western Blotting (WB)

Séquence: VDHLLPVDEN FSS-C

Specificité: This antibody detects KLF15 at N-term.

Réactivité croisée (Details): Species reactivity (expected):Mouse, Rat, Pig.
Species reactivity (tested):Human.

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogène: Peptide from N Terminus of the protein sequence according to NP_054798.1 Genename: KLF15

Information d'application

Indications d'application: Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.3-1.0 μg/mL. Approx 48 kDa band observed in lysates of hepatoblastomacell line HepG2 (calculated MW of 44.8 kDa according to NP_054798.1). Recommended(Data obtained with a previous EB05463 batch). Identical WB results were obtained with

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Détails sur KLF15

Antigène: KLF15 (Kruppel-Like Factor 15 (KLF15))

Autre désignation: KLF15 (KLF15 Produits)

Synonymes: anticorps KKLF, anticorps 1810013I09Rik, anticorps AV048136, anticorps AW494632, anticorps CKLF, anticorps Kruppel like factor 15, anticorps Kruppel-like factor 15 S homeolog, anticorps Kruppel-like factor 15, anticorps KLF15, anticorps klf15.S, anticorps Klf15

Sujet: The kidney chloride channel proteins CLCNKA and CLCNKB are highly related, although they are located in different areas of the kidney. Using a yeast 1-hybrid screen of a kidney cDNA library with the GA element of the CLCNKA promoter as bait, Uchida et al. (2000) isolated cDNAs encoding MAZ and KLF15, which they termed KKLF. Sequence analysis predicted that the 415-amino acid KLF15 protein, which is 84 % identical to the rat Klf15 protein, contains 3 zinc finger motifs at its C terminus, N-terminal serine-rich stretches, and a central proline-rich segment. EMSA analysis confirmed that KLF15 and MAZ interact with the GA element of the CLCNKA promoter and showed that KLF15 binds with higher affinity and is a functional competitor of MAZ. Northern blot analysis revealed highest expression of a 2.5-kb KLF15 transcript in liver, followed by heart, skeletal muscle, and kidney. No expression was found in bone marrow or lymphoid tissues. Western blot analysis showed expression of a 50-kD protein. Immunohistochemical analysis detected nuclear expression of KLF15 in liver sinusoid stellate cells, subcapsular fibroblasts, and portal fibroblasts. KLF15 was also expressed in cardiac and skeletal muscle interstitial cells and in kidney inner medulla, glomeruli, and cortical interstitium. Immunofluorescence microscopy, however, demonstrated no colocalization of KLF15 with CLCNKA.Synonyms: CKLF, Cardiovascular Krueppel-like factor, KKLF, KLF15, Kidney-enriched krueppel-like factor, Krueppel-like factor 15

ID gène: 28999

NCBI Accession: NP_054798

UniProt: Q9UIH9