FBXW2 anticorps (C-Term)

N° du produit ABIN374352

Détail du produit anti-FBXW2 anticorps

Antigène: FBXW2 (F-Box and WD Repeat Domain Containing 2 (FBXW2))

Épitope: C-Term

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien, Porc

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp FBXW2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Séquence: C-KRGSSFLAGE HPG

Specificité: This antibody detects FBXW2 / FBW2 / FWD2 at C-term.

Réactivité croisée (Details): Species reactivity (expected):Canine (Dog), Pig, Bovine.
Species reactivity (tested):Human, Mouse, Rat.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogène: Synthetic peptide from C-Terminus of the protein sequence according to NP_036296.1 Genename: FBXW2

Information d'application

Indications d'application: Peptide ELISA: Limit dilution 1: 128000. Western blot: 0.3-1.5 μg/mL. Approx 48 kDa band observed in Human Kidney lysates(calculated MW of 48.8 kDa according to NP_036296.1). A stronger band of unknownidentity was also consistently observed at appr.38 kDa - both bands were successfullyblocked by incubation with the immunising peptide. Immunohistochemistry: 2-3 μg/mL. In paraffin embedded Human Kidney, showscytoplasmic staining of epithelial cells in some renal tubules.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur FBXW2

Antigène: FBXW2 (F-Box and WD Repeat Domain Containing 2 (FBXW2))

Autre désignation: FBXW2 / FBW2 / FWD2 (FBXW2 Produits)

Synonymes: anticorps FBXW2, anticorps md6, anticorps fbw2, anticorps fwd2, anticorps MGC53244, anticorps FBW2, anticorps Fwd2, anticorps Md6, anticorps wu:fc72f10, anticorps zgc:110365, anticorps 2700071L08Rik, anticorps MD6, anticorps F-box and WD repeat domain containing 2, anticorps F-box and WD repeat domain containing 2 L homeolog, anticorps F-box and WD-40 domain protein 2, anticorps FBXW2, anticorps fbxw2, anticorps Fbxw2, anticorps fbxw2.L

Sujet: F box proteins are an expanding family of eukaryotic proteins characterized by an approximately 40 amino acid motif, the F box. Some F box proteins have been shown to be critical for the ubiquitin mediated degradation of cellular regulatory proteins. In fact, F box proteins are one of the four subunits of ubiquitin protein ligases, called SCFs. SCF ligases bring ubiquitin conjugating enzymes to substrates that are specifically recruited by the different F box proteins. A large family of mammalian F box proteins has recently been identified and classified into three groups based on the presence of either the WD 40 repeats, the leucine rich repeats, or the presence or absence of other protein protein interacting domains. The FBXW2 gene product, the second identified member of the F box gene family, contains multiple WD 40 repeats.Synonyms: F-box and WD-40 domain-containing protein 2, F-box/WD repeat-containing protein 2, Protein MD6

ID gène: 26190

NCBI Accession: NP_036296

UniProt: Q9UKT8