EIF4ENIF1 anticorps (C-Term)

N° du produit ABIN374390

Détail du produit anti-EIF4ENIF1 anticorps

Antigène: EIF4ENIF1 (Eukaryotic Translation Initiation Factor 4E Nuclear Import Factor 1 (EIF4ENIF1))

Épitope: C-Term

Reactivité: Humain, Souris, Rat, Porc, Boeuf (Vache), Chien

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp EIF4ENIF1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Séquence: C-AKVISVDELE YRQ

Specificité: This antibody is expected to recognise isoform 1 (NP_062817.2) and isoform b (NP_001157974.1).

Réactivité croisée (Details): Species reactivity (expected):Mouse, Rat, Dog, Pig and Cow.
Species reactivity (tested):Human.

Purification: Ammonium sulphate precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogène: Peptide from C Terminus of the protein sequence according to NP_062817.2, NP_001157973.1, NP_001157974.1. Genename: EIF4ENIF1

Information d'application

Indications d'application: Peptide ELISA: 1/32000. Western blot: 0.25-0.5 μg/mL. Approx 140-150 kDa band observed in 293 lysates (predictedsize of approx.100 kDa according to NP_062817.1, however our observation agrees withthat of Dostie et al (see Ref.1). Immunohistochemistry: 5-10 μg/mL. In paraffin embedded Human Pancreas shows strong staining of cytoplasm in acinar cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.

Détails sur EIF4ENIF1

Antigène: EIF4ENIF1 (Eukaryotic Translation Initiation Factor 4E Nuclear Import Factor 1 (EIF4ENIF1))

Autre désignation: EIF4ENIF1 / 4E-T (EIF4ENIF1 Produits)

Synonymes: anticorps RGD1560908, anticorps eif4enif1, anticorps MGC80355, anticorps EIF4ENIF1, anticorps wu:fd15a05, anticorps zgc:101646, anticorps MGC147579, anticorps 4E-T, anticorps Clast4, anticorps 2610509L04Rik, anticorps A930019J01Rik, anticorps AA410001, anticorps AU021239, anticorps D11Ertd166e, anticorps eukaryotic translation initiation factor 4E nuclear import factor 1, anticorps eukaryotic translation initiation factor 4E nuclear import factor 1 S homeolog, anticorps Eif4enif1, anticorps EIF4ENIF1, anticorps eif4enif1.S, anticorps eif4enif1

Sujet: EIF4ENIF1 is a nucleocytoplasmic shuttle protein for the translation initiation factor eIF4E. This shuttle protein mediates the nuclear import of eIF4E, by interacting with the importin alpha-beta complex. It is predominantly cytoplasmic, its own nuclear import is regulated by a nuclear localization signal and nuclear export signals.Synonyms: Eukaryotic translation initiation factor 4E nuclear import factor 1, Eukaryotic translation initiation factor 4E transporter, eIF4E transporter

ID gène: 56478

NCBI Accession: NP_062817

UniProt: Q9NRA8