Caspase 4 anticorps (Internal Region)

N° du produit ABIN374466

Cet anticorps anti-Caspase 4 Polyclonal Chèvre (ABIN374466) détecte spécifiquement Caspase 4 dans WB. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour Caspase 4 anticorps (Internal Region) (ABIN374466)

Antigène: Caspase 4 (CASP4) (Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Caspase 4 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-Caspase 4 anticorps

Épitope: Internal Region

Séquence: CKERAEEIYP IKERN

Specificité: This antibody is expected to recognise both isoforms of CASP4 (alpha, represented by NP_001216.1 and gamma, NP-150649.1).

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: Ammonium sulphate precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogène: Peptide from the internal region of the protein sequence according to NP_001216.1, NP_150649.1.

Information d'application

Indications d'application: Peptide ELISA: 1/16000. Western blot: 0.3-1.0 μg/mL. Approx 40 kDa band observed in Human Heart lysates(calculated MW of 43.0 kDa according to NP_001216.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.

Détails sur Caspase 4

Antigène: Caspase 4 (CASP4) (Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4))

Autre désignation: Caspase-4

Sujet: Caspase 4 is a 377 amino acid protein (43 kDa) that belongs to the Ced3/ICE family of cystein proteases. Caspase 4 is an "upstream" caspase with a long prodomain. It undergoes a cleavage upon activation, leading to p20 and p10 subunits which have 67 % homology to mature Caspase 1 over the equivalent coding sequences. Caspase 4 is capable of cleaving itself and the p30 Caspase 1 precursor, and of inducing apoptosis in transfected insect or COS cells. In high concentrations, it can also cleave the nuclear DNA enzyme PARP but the biological relevance of this cleavage is unclear. Caspase 4 mRNA is found in most tissues examined with the exception of the brain. Highest expression is found in spleen and lung, with moderate expression in heart and liver. Low expression is observed with skeletal muscle, kidney, and testis.Synonyms: CASP4, ICE(rel)-II, ICH2, Protease ICH-2, Protease TX

ID gène: 837, 9606

UniProt: P49662

Pathways: Apoptose, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity