RAE1 anticorps (C-Term)

N° du produit ABIN374586

Cet anticorps anti-RAE1 Polyclonal Chèvre (ABIN374586) détecte spécifiquement RAE1 dans WB, IHC (p) et EIA. L’anticorps est réactif avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour RAE1 anticorps (C-Term) (ABIN374586)

Antigène: RAE1 (RAE1 RNA Export 1 Homolog (S. Pombe) (RAE1))

Reactivité: Humain, Souris, Rat

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp RAE1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-RAE1 anticorps

Épitope: C-Term

Séquence: C-NAAEELKPRN KK

Specificité: This antibody recognizes RAE1.

Réactivité croisée (Details): Species reactivity (expected):Mouse, Rat, Canine, Porcine, Bovine.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogène: Peptide with sequence from the C Terminus of Human RAE1 Genename: RAE1

Information d'application

Indications d'application: Peptide ELISA: Detection Limit: 1/64000. Western Blot: 0.03-0.1 μg/mL. Detects a band of Approx 38-40 kDa in lysates of HumanHepatocarcinoma cell line HepG2.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur RAE1

Antigène: RAE1 (RAE1 RNA Export 1 Homolog (S. Pombe) (RAE1))

Autre désignation: RAE1

Sujet: Mutations in Rae1 and Gle2 (Saccharomyces cerevisae homolog) genes have been shown to result in accumulation of poly(A)-containing mRNA in the nucleus, suggesting that the encoded proteins are involved in RNA export. It contains four WD40 motifs, and has been shown to localize to distinct foci in the nucleoplasm, to the nuclear rim, and to meshwork-like structures throughout the cytoplasm. This gene is thought to be involved in nucleocytoplasmic transport, and in directly or indirectly attaching cytoplasmic mRNPs to the cytoskeleton. Depletion of Rae1 from extracts or cells severely inhibits mitotic spindle assembly. Alternatively spliced transcript variants encoding the same protein have been found for this gene.Synonyms: MRNP41, mRNA export factor, mRNA-associated protein mrnp 41

ID gène: 8480, 9606

UniProt: P78406

Pathways: SARS-CoV-2 Protein Interactome