Mre11 anticorps (AA 68-706)

N° du produit ABIN401319

Cet anticorps anti-Mre11 Polyclonal Lapin (ABIN401319) détecte spécifiquement Mre11 dans WB et EIA. L’anticorps est réactif avec des échantillons de Humain, Rat, Souris, Chien et Macaque.

Aperçu rapide pour Mre11 anticorps (AA 68-706) (ABIN401319)

Antigène: Mre11 (MRE11A) (MRE11 Meiotic Recombination 11 Homolog A (S. Cerevisiae) (MRE11A))

Reactivité: Humain, Rat, Souris, Chien, Macaque

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Mre11 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-Mre11 anticorps

Épitope: AA 68-706

Specificité: This antibody is directed against Mre11 protein.

Réactivité croisée (Details): Species reactivity (expected):Rat, Macaque, Human, Canine.
Species reactivity (tested):Mouse.

Purification: Affinity Purified

Immunogène: Recombinant protein corres-ponding to amino acids 68-706 of mouse Mre11 protein

Isotype: IgG

Information d'application

Indications d'application: ELISA: 1/10000 - 1/50000. Western Blot: 1/500 - 1/2000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.05 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Détails sur Mre11

Antigène: Mre11 (MRE11A) (MRE11 Meiotic Recombination 11 Homolog A (S. Cerevisiae) (MRE11A))

Autre désignation: MRE11

Sujet: Mre11 is a component of the MRN complex (Mre11/Rad50/Nbs1), which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses singlestrand endonuclease activity and doublestrand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation. Mre11 is a nuclear protein that is widely expressed and localizes to discrete nuclear foci after treatment with genotoxic agents (including irradiation). Alternative splice isoforms are recorded for this protein.Synonyms: Double-strand break repair protein MRE11A, HNGS1, MRE11 homolog 1, MRE11 meiotic recombination 11 homolog A, MRE11A

ID gène: 17535

NCBI Accession: NP_061206

UniProt: Q61216

Pathways: Réparation de l'ADN