ATR anticorps (Internal Region)

N° du produit ABIN401401

Cet anticorps Lapin Polyclonal détecte spécifiquement ATR dans WB et EIA. Il présente une réactivité envers Humain, Souris, Chien, Poisson, Singe, Rat et Xenopus laevis.

Aperçu rapide pour ATR anticorps (Internal Region) (ABIN401401)

Antigène: ATR (Ataxia Telangiectasia and Rad3 Related (ATR))

Reactivité: Humain, Souris, Chien, Poisson, Singe, Rat, Xenopus laevis

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ATR est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-ATR anticorps

Épitope: Internal Region

Specificité: This antibody is specific for ATR protein.

Réactivité croisée (Details): Species reactivity (expected):Mouse, Rat, Monkey, Canine, Fish, Xenopus.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogène: Synthetic peptide corresponding to an internal region of human ATR protein

Isotype: IgG

Information d'application

Indications d'application: ELISA: 1/15,000 - 1/70,000. Western Blot: 1/1,000 - 1/5,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.28 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Détails sur ATR

Antigène: ATR (Ataxia Telangiectasia and Rad3 Related (ATR))

Autre désignation: ATR / FRP1

Sujet: Ataxia Telangiectasia Mutated (ATM) and Rad 3-related protein (ATR) is a phosphatidylinositol kinase (PK)-related kinase which functions in response to DNA damage and repair as well as at DNA replication checkpoints during the cell cycle. ATR activates checkpoint signaling upon genotoxic stresses, such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. ATR is a member of the DNA-PK kinase family and is closely related to ATM and DNAPK for which DNA stimulates the observed kinase activity. Chromosomal remodeling proteins have also been reported to associate with ATR complexes, including histone deacetylases (HDAC1, HDAC2 and CHD4). ATR is known to phosphorylate BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and TP53/p53 which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. ATR is a nuclear protein, but can also be found in PML nuclear bodies in certain cell types. ATR is recruited to chromatin during Sphase and redistributes to discrete nuclear foci upon DNA damage, hypoxia or replication fork stalling.Synonyms: Ataxia telangiectasia and Rad3-related protein, FRAP-related protein 1, Serine/threonine-protein kinase ATR

ID gène: 545

NCBI Accession: NP_001175

UniProt: Q13535

Pathways: Signalisation p53, Apoptose, Réparation de l'ADN, Positive Regulation of Response to DNA Damage Stimulus