Mouse monoclonal antibody raised against native CD300A.
Specificité
This antibody reacts with CD300a, a 60 KDa leukocyte transmembrane glycoprotein expressed on human granulocytes, monocytes, neutrophils, NK cells, mast cells and dendritic cells, 25 % of circulating T cells and 15 % of circulating B cells.
Réactivité croisée
Humain
Immunogène
Native purified CD300A from human HPB acute lymphoid leukaemia cell line.
CD300A
Reactivité: Humain
WB
Hôte: Lapin
Polyclonal
PE-Cy3
Indications d'application
Flow Cytometry (20 µL in human blood cells 100 µL in whole blood or 106 cells in a suspension) The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Format
Liquid
Buffer
In PBS (0.2 % BSA, 0.09 % sodium azide)
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C
Stockage commentaire
Store in the dark at 4°C. Do not freeze. Avoid prolonged exposure to light. Aliquot to avoid repeated freezing and thawing.
Dimasi, Roessle, Moran, Candiano, Svergun, Biassoni: "Molecular analysis and solution structure from small-angle X-ray scattering of the human natural killer inhibitory receptor IRp60 (CD300a)." dans: International journal of biological macromolecules, Vol. 40, Issue 3, pp. 193-200, (2007) (PubMed).
Alvarez, Tang, Coligan, Borrego: "The CD300a (IRp60) inhibitory receptor is rapidly up-regulated on human neutrophils in response to inflammatory stimuli and modulates CD32a (FcgammaRIIa) mediated signaling." dans: Molecular immunology, Vol. 45, Issue 1, pp. 253-8, (2007) (PubMed).
Bachelet, Munitz, Levi-Schaffer: "Abrogation of allergic reactions by a bispecific antibody fragment linking IgE to CD300a." dans: The Journal of allergy and clinical immunology, Vol. 117, Issue 6, pp. 1314-20, (2006) (PubMed).