Apolipoprotein M anticorps (C-Term)

N° du produit ABIN452682

Détail du produit anti-Apolipoprotein M anticorps

Antigène: Apolipoprotein M (APOM)

Épitope: C-Term

Reactivité: Humain, Chien

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Apolipoprotein M est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificité: This antibody recognizes Apolipoprotein M (Apo M).

Réactivité croisée (Details): Species reactivity (expected):Canine.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogène: Peptide with sequence PRNQEACELSNN, from the C Terminus of the protein sequence according to NP_061974.2.

Information d'application

Indications d'application: Peptide ELISA: Antibody Detection Limit Dilution: 1/8000. Western Blot: Preliminary experiments gave an approx 50 kDa band in Human Kidney andLiver lysates after 0.2 μg/mL antibody staining. Please note that currently we cannot find anexplanation in the literature for the band we observe given the calculated size of 21.0 kDaaccording to NP_061974.2. We do note that ApoM is a glycoprotein which makes a highersize band to be expected. The 50 kDa band was successfully blocked by incubation withthe immunizing peptide. Immunohistochemistry: 3-6 μg/mL. In paraffin embedded Human Liver shows strong stain of cytoplasm in select hepatocytes.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Détails sur Apolipoprotein M

Antigène: Apolipoprotein M (APOM)

Autre désignation: Apolipoprotein M (Apo M) (APOM Produits)

Synonymes: anticorps G3a, anticorps HSPC336, anticorps NG20, anticorps apo-M, anticorps 1190010O19Rik, anticorps APOM, anticorps fb62d12, anticorps wu:fb62d12, anticorps g3a, anticorps ng20, anticorps hspc336, anticorps mKIAA4033, anticorps apolipoprotein M, anticorps apolipoprotein M S homeolog, anticorps myeloperoxidase, anticorps APOM, anticorps Apom, anticorps apom.S, anticorps apom, anticorps Mpo

Sujet: Apolipoprotein M is a member of the lipocalin protein family. It is found associated with high density lipoproteins and to a lesser extent with low density lipoproteins and triglyceride-rich lipoproteins. It is positively related to leptin. It is mainly expressed in liver and kidney. Apolipoprotein M is secreted through the plasma membrane but remains membrane-bound, where it is involved in lipid transport. It is important for the formation of prebeta-HDL and cholesterol efflux to HDL, and thereby inhibits formation of atherosclerotic lesions.Synonyms: Apo-M, ApoM, HSPC336, NG20, Protein G3A

ID gène: 55937

NCBI Accession: NP_061974

UniProt: O95445