Lapin anti-Mouton IgG (Fc Region), (Fab Region) Anticorps (Biotin)

Détails pour le produit réf. ABIN458773
Antigène
Épitope
Fc Region, Fab Region
1567
134
99
55
33
33
25
6
6
6
6
5
4
4
4
3
2
1
1
1
Reactivité
Mouton
355
341
336
232
232
121
112
87
80
73
72
65
61
57
54
49
43
31
27
15
9
8
7
5
4
4
4
4
4
3
3
3
3
2
2
1
1
1
1
Hôte
Lapin
842
798
335
169
93
84
23
22
13
5
4
4
4
3
1
1
1
1
Clonalité
Polyclonal
Conjugué
Biotin
236
219
208
149
147
130
75
49
48
35
31
26
26
26
25
25
25
23
23
23
22
22
21
21
21
20
20
20
20
20
20
18
15
14
13
10
9
9
9
9
8
8
6
6
6
6
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (IHC), ELISA
Options
Immunogène Purified normal IgG isolated from pooled sheep serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Isotype IgG
Specificité The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule.
Attributs du produit Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to sheep IgG, heavy and light chains
Biotin/IgG protein molar ratio (B/P) is approximately 4.2.
Purification No adsorption required. Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography.
Antigène
Indications d'application In immunocytochemical and immunohistochemical staining to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen or immune complex using a reference antibody of sheep origin in the middle layer of the indirect test procedure. As a second step an avidin or streptavidin conjugate of the user's choice has to be used. This immunoconjugate is not pre-diluted.
The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500, in ELISA and comparable non-precipitating antibody-binding assays between 1:1,000 and 1:10,000.
Restrictions For Research Use only
Format Lyophilized
Reconstitution It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -24 °C.
Concentration 10 mg/mL
Buffer Biotin-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).
Agent conservateur Without preservative
Conseil sur la manipulation Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
Stock 4 °C/-20 °C
Stockage commentaire The lyophilized conjugate is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.