Tetramethylrhodamine Isothiocyanate Isomer R anticorps

Détails pour le produit réf. ABIN458843
Clonalité (Clone)
Monoclonal ()
ELISA, Western Blotting (WB)
Immunogène Highly purified fluorescein isothiocyanate isomer 1.
Clone NI 308
Isotype IgG1 kappa
Specificité This antiserum has not been tested for cross-reactivity with other fluorescent compounds.
Attributs du produit Purified monoclonal mouse antibody to tetramethylrhodamine isothiocyanate isomer R
Purification Purified
Classe de substances Chemical
Sujet The reactivity of the antiserum is directed to the TRITC molecule as tested in direct binding enzyme immunoassay, ELISA and immunoblotting.
Indications d'application To enhance the specific signal obtained with a monoclonal antibody or a polyclonal second antibody conjugated to TRITC. The phenomenon of a weak reaction of a monoclonal antibody is e.g. well known in different analysis of vital peripheral blood mononuclear cells in suspensions by the expression of surface markers. A similar situation exists in solid phase assay systems (ELISA, blotting, DIBA) when used for the identification and /or quantitative determination of minute amounts of soluble specific antigens or antibodies. The sensitivity of the TRITC hapten-anti-hapten system makes it a valuable alternative to the biotin-avidin system. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:40, in ELISA from 1:100 upwards, in Western blotting from 1:200 upwards. These data should be interpreted as general recommendations only.
Restrictions For Research Use only
Format Lyophilized
Concentration IgG concentration is 0.4 mg/ml. No foreign proteins added.
Buffer Purified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline (pH 7.2).
Stock 4 °C/-20 °C
Stockage commentaire The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below - 20 °C. Reconstitute the lyophilized ascites by adding 0.5 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4 °C, not refrozen, and preferably used the same day.