FOXO3 anticorps (pThr24, Thr32)

N° du produit ABIN461993

L’anticorps Lapin Polyclonal anti-FOXO3 a été validé pour WB, IHC, ELISA, IHC (p) et IP. Il convient pour détecter FOXO3 dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour FOXO3 anticorps (pThr24, Thr32) (ABIN461993)

Antigène: FOXO3 (Forkhead Box O3 (FOXO3))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp FOXO3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Détail du produit anti-FOXO3 anticorps

Épitope: pThr24, Thr32

Marque: IHC-plus™

Specificité: Detects endogenous levels of FKHR (FKHRL1) only when activated by phosphorylation at Thr24 of FKHR or Thr32 of FKHRL1. Also reacts with phosphorylated AFX at Thr28. Does not react with FKHR family members phosphorylated at other sites. Species cross-reactivity: Human, mouse, rat.

Purification: Protein A purified

Immunogène: Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Thr24 of human FKHR.

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: ELISA, IHC, IHC-P (1:100), IP (1:50), WB (1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:100.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur FOXO3

Antigène: FOXO3 (Forkhead Box O3 (FOXO3))

Autre désignation: FOXO3 / FOXO3A

Sujet: Name/Gene ID: FOXO3
Family: Transcription factor

Synonyms: FOXO3, AF6q21, AF6q21 protein, Forkhead box O3, Forkhead box O3A, FOXO3A, FKHRL1P2, Forkhead box protein O3, FOXO2, FKHRL1

ID gène: 2309

UniProt: O43524

Pathways: Cycle Cellulaire, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis