PPP4C anticorps (AA 290-301)

N° du produit ABIN462300

Détail du produit anti-PPP4C anticorps

Antigène: PPP4C (Protein Phosphatase 4, Catalytic Subunit (PPP4C))

Épitope: AA 290-301

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien, Lapin, Cheval, Poisson zèbre (Danio rerio), Roussette (Chauve-souris), Hamster, Singe, Xenopus laevis

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp PPP4C est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Human PPP4C.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Opossum, Platypus, Xenopus, Catfish, Stickleback, Pufferfish, Zebrafish, Water flea (100%) Drosophila, Mosquito, Ant, Beetle (92%) Seq squirt, Poplar, Trichoplax, Toxoplasma, Tick, Spikemoss, Amoeba, Neospora (83%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide C-PQETRGIPSKKP from the C-terminus of human PPP4C (NP_002711.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Opossum, Platypus, Xenopus, Catfish, Stickleback, Pufferfish, Zebrafish, Water flea (100%), Drosophila, Mosquito, Ant, Beetle (92%), Seq squirt, Poplar, Trichoplax, Toxoplasma, Tick, Spikemoss, Amoeba, Neospora (83%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA, WB (0.1 - 0.3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL. Western Blot: Approximately 35kD band observed in HEK293, A431 and HeLa lysates (calculated MW of 35.1kD according to NP_002711.1).

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur PPP4C

Antigène: PPP4C (Protein Phosphatase 4, Catalytic Subunit (PPP4C))

Autre désignation: PPP4C (PPP4C Produits)

Synonymes: anticorps pp4, anticorps ppx, anticorps pp4c, anticorps pph3, anticorps MGC75928, anticorps PP4C-A, anticorps gc:56413, anticorps ppp4c, anticorps wu:fd05e08, anticorps zgc:56413, anticorps PP4, anticorps PP4C, anticorps PPH3, anticorps PPP4, anticorps PPX, anticorps 1110002D08Rik, anticorps AU016079, anticorps Ppx, anticorps protein phosphatase 4 catalytic subunit, anticorps protein phosphatase 4, catalytic subunit a, anticorps protein phosphatase 4, catalytic subunit, anticorps protein phosphatase 4 catalytic subunit S homeolog, anticorps ppp4c, anticorps ppp4ca, anticorps PPP4C, anticorps Ppp4c, anticorps ppp4c.S

Sujet: Name/Gene ID: PPP4C
Subfamily: Protein Phosphatase - Serine/Threonine other
Family: Protein Phosphatase

Synonyms: PPP4C, PP4, PPH3, Protein phosphatase X, PP-X, Protein phosphatase 4, PP4C, PPP4, PPX

ID gène: 5531

NCBI Accession: NP_002711

UniProt: P60510