VEGFA anticorps (Biotin)

N° du produit ABIN465294

L’anticorps anti-VEGFA Polyclonal Lapin est utilisé pour la détection de VEGFA dans des échantillons de Souris. Il a été validé pour WB, ELISA et Neut.

Aperçu rapide pour VEGFA anticorps (Biotin) (ABIN465294)

Antigène: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp VEGFA est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Détail du produit anti-VEGFA anticorps (Biotin)

Specificité: Mouse VEGF

Purification: Immunoaffinity purified

Immunogène: E.coli derived recombinant Murine VEGF.

Type of Immunogen: Cell extract

Information d'application

Indications d'application: Approved: ELISA (1 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect mVEGF by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mVEGF. To detect mVEGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Murine VEGF (LS-C104513) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mVEGF. Western Blot: To detect mVEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mVEGF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Commentaires:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Conseil sur la manipulation: Avoid freeze/thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Détails sur VEGFA

Antigène: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Autre désignation: VEGFA / VEGF

Sujet: Name/Gene ID: VEGFA
Family: PDGF

Synonyms: VEGFA, VPF, Vascular permeability factor, VEGF, VEGF-A, MVCD1

ID gène: 7422

UniProt: P15692

Pathways: Signalisation RTK, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling