RANKL anticorps

N° du produit ABIN465771

Cet anticorps anti-RANKL Polyclonal Lapin (ABIN465771) détecte spécifiquement RANKL dans WB et ELISA. L’anticorps est réactif avec des échantillons de Souris.

Aperçu rapide pour RANKL anticorps (ABIN465771)

Antigène: RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RANKL est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-RANKL anticorps

Specificité: Mouse sRANKL

Purification: Immunoaffinity purified

Immunogène: E.coli derived recombinant Murine sRANKL.

Type of Immunogen: Cell extract

Information d'application

Indications d'application: Approved: ELISA (0.5 - 2 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of msRANKL (50 ng/mL), a concentration of 05 μg/mL of this antibody is required. ELISA: To detect msRANKL by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine sRANKL (LS-C104817) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant msRANKL. Western Blot: To detect msRANKL by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant msRANKL is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Commentaires:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Conseil sur la manipulation: Avoid freeze/thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Détails sur RANKL

Antigène: RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))

Autre désignation: TNFSF11 / RANKL / TRANCE

Sujet: Name/Gene ID: TNFSF11
Family: TNF

Synonyms: TNFSF11, CD254, CD254 antigen, HRANKL2, OPTB2, TRANCE, ODF, OPGL, SOdf, Osteoprotegerin ligand, RANKL

ID gène: 8600

UniProt: O14788

Pathways: Signalisation NF-kappaB