KIR2DL4/CD158d anticorps (PE)

N° du produit ABIN489933

Détail du produit anti-KIR2DL4/CD158d anticorps (PE)

Antigène: KIR2DL4/CD158d (KIR2DL4) (Killer Cell Immunoglobulin-Like Receptor, Two Domains, Long Cytoplasmic Tail, 4 (KIR2DL4))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp KIR2DL4/CD158d est conjugé à/à la PE

Application: Flow Cytometry (FACS)

Specificité: The mouse monoclonal antibody mAb#33 (also known as mAb 33 or 33) recognizes extracellular portion of CD158d / KIR2DL4, a 45 kDa NK cell marker. Cell surface expression and function of CD158d / KIR2DL4 depends on genotype of particular individuals.

Réactivité croisée (Details): Human

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogène: NK3.3 cells and KIR2DL4-Ig fusion protein

Clone: MAb 33

Isotype: IgG1 kappa

Information d'application

Indications d'application: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Commentaires:

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Restrictions: For Research Use only

Stockage

Reconstitution: No reconstitution is necessary.

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.
Avoid prolonged exposure to light.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-KIR2DL4/CD158d (PE) (ABIN489933)

Rajagopalan, Bryceson, Kuppusamy, Geraghty, van der Meer, Joosten, Long: "Activation of NK cells by an endocytosed receptor for soluble HLA-G." dans: PLoS biology, Vol. 4, Issue 1, pp. e9, (2006) (PubMed).

LeMaoult, Zafaranloo, Le Danff, Carosella: "HLA-G up-regulates ILT2, ILT3, ILT4, and KIR2DL4 in antigen presenting cells, NK cells, and T cells." dans: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 19, Issue 6, pp. 662-4, (2005) (PubMed).

Yan, Fan: "Residues Met76 and Gln79 in HLA-G alpha1 domain involve in KIR2DL4 recognition." dans: Cell research, Vol. 15, Issue 3, pp. 176-82, (2005) (PubMed).

Goodridge, Witt, Christiansen, Warren: "KIR2DL4 (CD158d) genotype influences expression and function in NK cells." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 4, pp. 1768-74, (2003) (PubMed).

Rajagopalan, Fu, Long: "Cutting edge: induction of IFN-gamma production but not cytotoxicity by the killer cell Ig-like receptor KIR2DL4 (CD158d) in resting NK cells." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 4, pp. 1877-81, (2001) (PubMed).

Détails sur KIR2DL4/CD158d

Antigène: KIR2DL4/CD158d (KIR2DL4) (Killer Cell Immunoglobulin-Like Receptor, Two Domains, Long Cytoplasmic Tail, 4 (KIR2DL4))

Autre désignation: CD158d / KIR2DL4 (KIR2DL4 Produits)

Synonymes: anticorps G9P, anticorps CD158D, anticorps KIR103, anticorps KIR103AS, anticorps KIR2DL4, anticorps killer cell immunoglobulin like receptor, two Ig domains and long cytoplasmic tail 4, anticorps killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 4, anticorps KIR2DL4

Sujet: Killer cell immunoglobulin like receptor, two Ig d,CD158d / KIR2DL4 is a KIR family member that shares structural features with both activating and inhibitory receptors and may mediate different functions under different circumstances. It contains cytoplasmic ITIM, suggesting inhibitory function, but also transmembrane domain similar to those of activating KIRs. It has been reported that CD158d serves as an inhibitory receptor for peripheral and uterine NK cells, but its ligation with soluble mAbs (unlike immobilized mAbs) results in activation of IFN-γ, secretion. CD158d also binds both membrane form and soluble form of its ligand HLA-G.,KIR2DL4, KIR103AS, 103AS, 15.212

ID gène: 3805

UniProt: Q99706