PTK6 anticorps

N° du produit ABIN492612

Cet anticorps anti-PTK6 est un anticorps Souris Monoclonal détectant PTK6 dans WB. Adapté pour Humain.

Aperçu rapide pour PTK6 anticorps (ABIN492612)

Antigène: PTK6 (PTK6 Protein tyrosine Kinase 6 (PTK6))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PTK6 est non-conjugé

Application: Western Blotting (WB)

Clone: 24B7

Détail du produit anti-PTK6 anticorps

Specificité: Recognizes BRK/PTK6

Attributs du produit: Synonyms: Tyrosine-protein kinase 6, Breast tumor kinase, Tyrosine-protein kinase BRK

Purification: Subsequent Ultrafiltration and Size Exclusion Chromatography.

Immunogène: Peptide conjugated to hemocyanin. Epitope: Internal peptide derived from BRK/PTK6 sequence

Isotype: IgG1

Information d'application

Indications d'application: Immunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Included Potitive Control: Cell lysate from untreated MDA-MB 468 cells (See Protocols formore details).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocole: Positive Control: Cell lysate from untreated MDA-MB-468 cells, breast adenocarcinoma(Human)Format: Lyophilized cell lysate from serum starved MDA-MB-468 cells. Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20. 000 cells. Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended forapplications with native proteins such as in immunoprecipitation.

Restrictions: For Research Use only

Stockage

Concentration: 0.1 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide, PEG, Sucrose and 50 % Glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store the antibody in aliquots) at -20 °C. Avoid repeated freeze-thaw cycles.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur PTK6

Antigène: PTK6 (PTK6 Protein tyrosine Kinase 6 (PTK6))

Autre désignation: PTK6 / BRK

Sujet: This protein encoded is a cytoplasmic nonreceptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Overexpression of this gene in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Expression of this gene has been detected at low levels in some breast tumors but not in normal breast tissue. Its presence in the nucleus appears to be linked to suppression of tumor progression. The encoded protein has been shown to undergo autophosphorylation.Synonyms: Breast tumor kinase, Tyrosine-protein kinase 6, Tyrosine-protein kinase BRK

ID gène: 5753

UniProt: Q13882